The Role of Particulate Matter-Associated Zinc in Cardiac Injury in Rats

Details

• Personal Author:
  Christiani, David C. ; Costa DL ; Gilmour PS ; Jaskot R ; Karoly ED ; Kodavanti UP ; Ledbetter AD ; Madamanchi NR ; Mandavilli BS ; Nyska A ; Peddada S ; Richards JH ; Runge MS ; Schladweiler MC ; Thomas R ; Wallenborn JG
• Description:
  BACKGROUND: Exposure to particulate matter (PM) has been associated with increased cardiovascular morbidity; however, causative components are unknown. Zinc is a major element detected at high levels in urban air. OBJECTIVE: We investigated the role of PM-associated zinc in cardiac injury. METHODS: We repeatedly exposed 12- to 14-week-old male Wistar Kyoto rats intratracheally (1x/week for 8 or 16 weeks) to a) saline (control); b) PM having no soluble zinc (Mount St. Helens ash, MSH); or c) whole-combustion PM suspension containing 14.5 microg/mg of water-soluble zinc at high dose (PM-HD) and d ) low dose (PM-LD), e) the aqueous fraction of this suspension (14.5 microg/mg of soluble zinc) (PM-L), or f ) zinc sulfate (rats exposed for 8 weeks received double the concentration of all PM components of rats exposed for 16 weeks). RESULTS: Pulmonary inflammation was apparent in all exposure groups when compared with saline (8 weeks > 16 weeks). PM with or without zinc, or with zinc alone caused small increases in focal subepicardial inflammation, degeneration, and fibrosis. Lesions were not detected in controls at 8 weeks but were noted at 16 weeks. We analyzed mitochondrial DNA damage using quantitative polymerase chain reaction and found that all groups except MSH caused varying degrees of damage relative to control. Total cardiac aconitase activity was inhibited in rats receiving soluble zinc. Expression array analysis of heart tissue revealed modest changes in mRNA for genes involved in signaling, ion channels function, oxidative stress, mitochondrial fatty acid metabolism, and cell cycle regulation in zinc but not in MSH-exposed rats. CONCLUSION: These results suggest that water-soluble PM-associated zinc may be one of the causal components involved in PM cardiac effects. [Description provided by NIOSH]
• Subjects:
  Air Pollution Animals Cardiovascular Diseases Cardiovascular System Chemistry Energy Metabolism Environmental Exposure Fibrosis Genetics Hazardous Substances Laboratories Lung Lung Diseases Morbidity Occupational Health Particulate Matter Pathology Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety

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• Keywords:
  Air Pollution Author Keywords: Aconitase Cardiac Function Cardiac Gene Expression Profile Cardiovascular System Disease Cardiovascular System Disorders Chemical Composition Chemical Reactions Chemicals Deoxyribonucleic Acids DNA DNA Damage Exposure Levels Genes Injuries Laboratory Animals Lung Disorders Metabolism Mitochondria Morbidity Rates Oxidative Stress Particulate Matter Particulates Pulmonary System Disorders Risk Factors Toxins Zinc Zinc And Compounds

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• ISSN:
  0091-6765
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; North Carolina ; OSHA Region 1 ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  13-20
• Volume:
  116
• Issue:
  1
• NIOSHTIC Number:
  nn:20057345
• Citation:
  Environ Health Perspect 2008 Jan; 116(1):13-20
• Contact Point Address:
  Urmila P. Kodavanti, National Health and Environmental Effects Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, NC 27710
• Email:
  kodavanti.urmila@epa.gov
• CAS Registry Number:
  Zinc (CAS RN 7440-66-6)
• Federal Fiscal Year:
  2008
• Performing Organization:
  Harvard School of Public Health
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Environmental Health Perspectives
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:69910d17a4c7e9d5936b8ad83a1993cdf30ec4914ce7c4a15238a2f6e707a6a98c982cdd9ed7a955fad35b591dd41ac4fef8c542ad679fe498b8c76e2646d4c8
• Download URL:
  https://stacks.cdc.gov/view/cdc/214729/cdc_214729_DS1.pdf
• File Type:
  [PDF - 372.86 KB ]