Comparison of Methods to Assess Dietary Exposure to Polycyclic Aromatic Hydrocarbons and Heterocyclic Amines

Details

• Personal Author:
  Deziel NC
• Description:
  Background. Studies of exposure to polycyclic aromatic hydrocarbons (PAH) and heterocyclic amines (HCA), carcinogens found in cooked meats, have observed an increased risk of cancer, but results have been inconsistent. The standard practice is to use food frequency questionnaires (FFQ) and carcinogen databases to estimate dietary intake of these chemicals, yet little is known about how these methods compare to other available methods, particularly biological markers. Methods. We compared exposure estimates for the PAH benzo[a]pyrene (BaP) and pyrene and the HCA 2-amino-1-methyl-6-phenylimidazo[4,5b]pyridine (PhIP) derived from the following methods: a meat-specific FFQ administered at twice and combined with a dietary carcinogen database; diet diaries longitudinally collected three times, combined with the same database; and urinary PhIP and 1-hydroxypyrene-glucuronide (1-OHPG) measured in overnight urine samples longitudinally collected at three times concurrent with the diaries. This study was completed in 54 non-smoking controls from an ongoing case-control study of colorectal adenoma. Results. PAH exposure estimates were log-normally distributed with means +/- standard deviations of 25.4 +/- 67.3 ng/day for the diary-database; 18.3 +/- 22.9 ng/day for the FFQ-database; and 157.6 +/- 200.7 ng/day for urinary 1-OHPG. Grains contributed more to total BaP intake compared to meats in 60% of participants. The strongest between-method association was observed between the FFQ-database derived BaP estimates and urinary 1-OHPG measurements (p<0.001). The diet diary-derived BaP was also statistically significantly associated with urinary 1-OHPG after adjusting for cotinine and when including both meat and grain sources (p=0.03). Statistically significant correlations between 1-OHPG and both the FFQ and diet diaries were observed when using daily meat intake without using the database (FFQ: r=0.31, p=0.04; diaries r=0.33, p=0.03). HCA exposure estimates were log-normally distributed with means +/- standard deviations of 68.6 +/- 78.7 ng/day for FFQ-database; 92.9 +/- 152.4 ng/day for diet diaries-database; and 29.5 +/- 39.5 ng/day for PhIP intake estimated by urinary PhIP. The majority of participants had no dietary PhIP exposure based on the diaries-database. The only statistically significant between-method association was between the diet diary-database derived PhIP estimates and urinary PhIP, when restricting the analyses to values above zero or the detection limit (regression p=0.02; Spearman r=0.81, p<0.001). Conclusions. The three different approaches for estimating dietary exposure to PAH were moderately correlated, providing confidence in the different methods. In contrast, the FFQ-database derived PhIP estimates were not statistically associated with either the diary-database derived estimates or the urinary PhIP. This may be due to the small number of sampling days or short-comings of the FFQ and the database. This study highlights the need to improve and validate methods for dietary exposure assessment. [Description provided by NIOSH]
• Subjects:
  Carcinogens Hydrocarbons Neoplasms Occupational Health Polycyclic Aromatic Hydrocarbons Safety
• Keywords:
  Cancer Rates Colorectal Cancer Dietary Effects Exposure Assessment Food Additives Heterocyclic Compounds Polycyclic Aromatic Hydrocarbons
• ISBN:
  9780549579670
• Publisher:
  ProQuest LLC.
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Dissertation
• Place as Subject:
  Maryland ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20056959
• Citation:
  Ann Arbor, MI: ProQuest LLC., 2008 Jan; :3309637
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8)
• Federal Fiscal Year:
  2008
• Performing Organization:
  Johns Hopkins University
• Peer Reviewed:
  False
• Start Date:
  20050701
• Source Full Name:
  Comparison of methods to assess dietary exposure to polycyclic aromatic hydrocarbons and heterocyclic amines
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4bc90a3bf82a12afb2b3d18a71c7f682cf66597ba61787b6483f7348a0f5cc6e50738161820da299e5a35f643e70c3cc638f78168c0916c643e8b11c2e47b754
• Download URL:
  https://stacks.cdc.gov/view/cdc/214447/cdc_214447_DS1.pdf
• File Type:
  [PDF - 9.47 MB ]