Organic Dust Inhibits Surfactant Protein Expression by Reducing Thyroid Transcription Factor-1 Levels in Human Lung Epithelial Cells

Details

• Personal Author:
  Boggaram V ; Gangam K ; Gottipati KR ; Meganathan V ; Mitchell C ; Natarajan K
• Description:
  Exposure to organic dust is a risk factor for the development of respiratory diseases. Surfactant proteins (SP) reduce alveolar surface tension and modulate innate immune responses to control lung inflammation. Therefore, changes in SP levels could contribute to the development of organic-dust-induced respiratory diseases. Because information on the effects of organic dust on SP levels is lacking, we studied the effects of dust from a poultry farm on SP expression. We found that dust extract reduced SP-A and SP-B mRNA and protein levels in H441 human lung epithelial cells by inhibiting their promoter activities, but did not have any effect on SP-D protein levels. Dust extract also reduced SP-A and SP-C levels in primary human alveolar epithelial cells. The inhibitory effects were not due to LPS or protease activities present in dust extract or mediated via oxidative stress, but were dependent on a heat-labile factor(s). Thyroid transcription factor-1, a key transcriptional activator of SP expression, was reduced in dust-extract-treated cells, indicating that its down-regulation mediates inhibition of SP levels. Our study implies that down-regulation of SP levels by organic dust could contribute to the development of lung inflammation and respiratory diseases in humans. [Description provided by NIOSH]
• Subjects:
  Dust Food Handling Food Industry Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Lung Surfactant Lung Cells Lung Epithelial Cells Lung Inflammation Organic Dust Organic Dusts Poultry Workers Respirable Dust Respiratory Diseases
• ISSN:
  1753-4259
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  118-131
• Volume:
  25
• Issue:
  2
• NIOSHTIC Number:
  nn:20056811
• Citation:
  Innate Immun 2019 Feb; 25(2):118-131
• Contact Point Address:
  Vijay Boggaram, Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708-3154, USA
• Email:
  vijay.boggaram@uthct.edu
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Texas Health Center at Tyler
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Innate Immunity
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:852d1de1a8adb679a0a4f601816181e99e1055cf4bfe8230f52603edd8cc96bceccc7483d67c87c653e6e7228213011253b72e981760ce4982c225a081f9d01b
• Download URL:
  https://stacks.cdc.gov/view/cdc/214325/cdc_214325_DS1.pdf
• File Type:
  [PDF - 857.87 KB ]