Carbon Disulfide Inhalation Increases Ca2+/Calmodulin-Dependent Kinase Phosphorylation of Cytoskeletal Proteins in the Rat Central Nervous System

Details

• Personal Author:
  Abou-Donia MB ; Viana ME ; Wilmarth KR
• Description:
  The Ca2+/calmodulin-dependent phosphorylation of neuronal cytoskeletal proteins was studied in brain supernatants prepared from rats exposed via inhalation to 600 or 800 ppm carbon disulfide (CS2) for 14 days. Exposure to CS2 resulted in increased phosphorylation of endogenous MAP-2 and exogenously added neurofilament triplet proteins. There also was an observed increase in the autophosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II). Slight increases in the binding of a monoclonal antibody to the a subunit of CaM kinase II were seen, while large increases in the binding of [125Icalmodulin to the a subunit of CaM kinase II also were observed. The finding of large increases in the autophosphorylation and calmodulin-binding to CaM kinase II with only slight increases in the amount of antibody-binding suggests that CS2 exposure results in increased Ca2+/calmodulin-dependent phosphorylation of proteins by inducing an increase in kinase activity. [Description provided by NIOSH]
• Subjects:
  Animals Occupational Health Respiratory System Safety
• Keywords:
  Animal Studies Author Keywords: Carbon Disulfide CaM Kinase II Central Nervous System Cytoskeletal Protein Inhalation Studies Phosphorylation Proteins
• ISSN:
  0006-8993
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  293-300
• Volume:
  628
• Issue:
  1
• NIOSHTIC Number:
  nn:20056722
• Citation:
  Brain Res 1993 Nov; 628(1-2):293-300
• Contact Point Address:
  Mohamed B. Abou-Donia, Department of Pharmacology, Duke University Medical Center, Durham, NC 27710, USA
• CAS Registry Number:
  Carbon disulfide (CAS RN 75-15-0)
• Federal Fiscal Year:
  1994
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Brain Research
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a47482c4e7bef235ee29416a3e04828df03531072bdbbeb01e01709c92d87cc58c4ee06c94ca62dbaa7280ecf7766057cd878fe91743f84d2e1e81ccfe201ea3
• Download URL:
  https://stacks.cdc.gov/view/cdc/214274/cdc_214274_DS1.pdf
• File Type:
  [PDF - 2.28 MB ]