DNA Damage in Human Sperm Is Related to Urinary Levels of Phthalate Monoester and Oxidative Metabolites

Details

• Personal Author:
  Calafat, Antonia M. ; Duty S ; Hauser R ; Meeker JD ; Ryan L ; Silva MJ ; Singh NP
• Description:
  BACKGROUND: The ubiquitous use of phthalate esters in plastics, personal care products and food packaging materials results in widespread general population exposure. In this report, we extend our preliminary study on the relationship between urinary concentrations of phthalate metabolites and sperm DNA damage among a larger sample of men and include measurements of mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) and mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP), two oxidative metabolites of di-(2-ethylhexyl) phthalate (DEHP). METHODS: Among 379 men from an infertility clinic, urinary concentrations of phthalate metabolites were measured using isotope-dilution high-performance liquid chromatography-tandem mass spectrometry. Sperm DNA damage measurements, assessed with the neutral comet assay, included comet extent (CE), percentage of DNA in tail (Tail%) and tail distributed moment (TDM). RESULTS: Monoethyl phthalate (MEP), a metabolite of diethyl phthalate, was associated with increased DNA damage, confirming our previous findings. Mono-(2-ethylhexyl) phthalate (MEHP), a metabolite of DEHP, was associated with DNA damage after adjustment for the oxidative DEHP metabolites. After adjustment for MEHHP, for an interquartile range increase in urinary MEHP, CE increased 17.3% [95% confidence interval (CI) = 8.7-25.7%], TDM increased 14.3% (95% CI = 6.8-21.7%) and Tail% increased 17.5% (95% CI = 3.5-31.5%). CONCLUSIONS: Sperm DNA damage was associated with MEP and with MEHP after adjusting for DEHP oxidative metabolites, which may serve as phenotypic markers of DEHP metabolism to 'less toxic' metabolites. The urinary levels of phthalate metabolites among these men were similar to those reported for the US general population, suggesting that exposure to some phthalates may affect the population distribution of sperm DNA damage. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Environmental Exposure Men Occupational Health Phthalic Acids Risk Assessment Risk Factors Safety Urine Urogenital System
• Keywords:
  Author Keywords: Phthalates Comet Assay Deoxyribonucleic Acids DNA DNA Damage Exposure Levels Fertility Humans Human Sperm Mass Spectrometry Metabolites Phthalates Reproductive Effects Risk Factors Urinalysis Urinary Metabolites Urine Chemistry

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• ISSN:
  0268-1161
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  22
• Issue:
  3
• NIOSHTIC Number:
  nn:20056539
• Citation:
  Hum Reprod 2007 Mar; 22(3):688-695
• Contact Point Address:
  Russ Hauser, Department of Environmental Health, Environmental and Occupational Medicine and Epidemiology, Harvard School of Public Health, 665 Huntington Avenue, Building 1, room 1405, Boston, MA 02115
• Email:
  hauser@hohp.harvard.edu
• CAS Registry Number:
  Dioctyl phthalate (CAS RN 117-81-7) ; Monoethyl phthalate (CAS RN 2306-33-4)
• Federal Fiscal Year:
  2007
• Performing Organization:
  Harvard School of Public Health
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Human Reproduction
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:02622d1cfe92142152658be64ecf682db426a77f5cd8ec533c7d774189dd18fcc47ab2dde13a2bdd121526ee0d72529dd555a9e3dac605ba5027c1580140dae8
• Download URL:
  https://stacks.cdc.gov/view/cdc/214130/cdc_214130_DS1.pdf
• File Type:
  [PDF - 130.43 KB ]