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Using QPCR for Rapid Quantification of Occupational Exposures to Brucella abortus



Details

  • Personal Author:
    Gruber ML
  • Description:
    Brucella abortus is the causative agent of the zoonotic disease Brucellosis. B. abortus is currently found within the Yellowstone ecosystem, which allows for the transmission of the pathogenic agent from wild bison and elk to domestic cattle populations. The transmission between animals has created the possibility of occupational exposures to humans through inhalation and contact with the bacterium. Current industrial hygiene bacterial monitoring methods consist of air sampling onto a culture plate or air samples collected within various types of filter cassettes, which are then analyzed through culturing methods. Culturing techniques are labor intensive and unreliable in quantifying exposure concentrations, as they are dependent on the ability to maintain cell viability. Quantitative Polymerase Chain Reaction (QPCR) has been used to quantify bacterial exposures through non-culture based methods. The use of a biological air sampling impingement system (SKC BioSampler(R)) was coupled with QPCR as methodologies for quantifying occupational exposures to B. abortus. The reliability of the sampling system was determined by aerosolizing B. abortus DNA at concentrations of 1X, 1.5X and 30X. All concentrations were air sampled with an SCK BioSampler(R) in triplicate and analyzed by QPCR. The reliability was determined within sample groupings by comparing mean concentrations. Results show that there was no significant difference in the quantification means when sampling with a SKC impingement system and analyzing through QPCR; at concentrations 1X and 30X. Significance was found within the concentration sample 1.5X, due to variance within the grouping sample 1.5X-2. In Addition, the quantified concentrations detected increased as the concentration nebulized increased. This research project was a feasibility study using QPCR and a SKC BioSampler(R) to quantify occupational exposures to B. abortus. Methods described within this research could prove to be a valuable tool for quantifying occupational B. abortus exposures and may contribute towards the understanding of quantifying bioaerosols. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • Publisher:
    Montana Tech of the University of Montana
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Thesis
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    1-58
  • NIOSHTIC Number:
    nn:20056160
  • Citation:
    Butte, MT: Montana Tech of the University of Montana, 2012 Dec; :1-58
  • Federal Fiscal Year:
    2013
  • Performing Organization:
    Montana Technological University
  • Peer Reviewed:
    False
  • Start Date:
    20050701
  • Source Full Name:
    Using QPCR for rapid quantification of occupational exposures to Brucella abortus
  • End Date:
    20290630
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:b3d75d67e8a7e80ccb8c377c4873bf9b0fface0401472f2ec3feee8fc86414a4651eb44f152e2fc37b13de886ac367f30941d6b93b66344ff000afee467a20d4
  • Download URL:
  • File Type:
    Filetype[PDF - 1.24 MB ]
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