A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies

Details

• Personal Author:
  Cui L ; He Z ; Imam SZ ; Konak T ; Liachenko S ; Miller DB ; O'Callaghan JP ; Panos J ; Paule MG ; Raymick J
• Description:
  Sampling and analysis of cerebrospinal fluid (CSF) is a common clinical practice used in the diagnosis, treatment, and prevention of neurological diseases. A similar interest is the sampling of CSF from rats to bridge the gap between bench-to-bedside work and to foster the development of new CSF biomarkers for clinical use. Here, we describe an improved procedure with an instrument designed in-house, by which rat CSF was successfully collected with indiscernible blood contamination (via the naked eye/surgical microscope amplification). The sampled CSF amounts were over 100 µl regardless of the animal's body weight, hydration status, and symptoms of systemic damage including, but not limited to, seizure, delusion (such as repeated hemorrhagic self-biting), hematuria, and gastrointestinal bleeding. In adult Sprague-Dawley rats above 300 g, the sampled CSF amounts were reliably at 200 µl or above with this method. There were no deaths related to the CSF sampling procedure. In conclusion, the present method provides a reliable and reproducible approach for collecting 200 µl CSF in rats without blood contamination. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Blood Eye Laboratories Occupational Health Safety Sense Organs
• Keywords:
  Author Keywords: Cerebrospinal Fluid Cerebrospinal Fluid Eyes In-house Designed Instrument Laboratory Animals Microscopy Neurological Diseases Rat Sampling
• ISBN:
  9781493992270
• ISSN:
  0893-2336
• Publisher:
  Humana Press
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  New York ; OSHA Region 10 ; OSHA Region 2 ; OSHA Region 3 ; Washington ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  233-243
• Volume:
  145
• NIOSHTIC Number:
  nn:20055943
• Citation:
  Cell culture techniques, neuromethods, second edition. Aschner M, Costa L, eds. New York: Humana Press, 2019 May; 145:233-243
• Contact Point Address:
  L.T. Michalovicz, Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Morgantown, WV
• Email:
  yqp4@cdc.gov
• Editor(s):
  Aschner M; Costa L
• Federal Fiscal Year:
  2019
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  Cell culture techniques, neuromethods, second edition
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d8563822a7f625e6836ab0aca83247b8462b1835b3d307e5df91433593cd85c534eccfaa4040c727af3d90fc14cbc1aef0c5038faf727ea97621fdb1383ade3c
• Download URL:
  https://stacks.cdc.gov/view/cdc/213691/cdc_213691_DS1.pdf
• File Type:
  [PDF - 522.20 KB ]