Characterization of Electronic Cigarette Aerosol and Its Induction of Oxidative Stress Response in Oral Keratinocytes

Details

• Personal Author:
  Chang CH ; Hu S ; Ji EH ; Messadi D ; Shu S ; Sun B ; Xia, T. ; Zhao T ; Zhu Y
• Description:
  In this study, we have generated and characterized Electronic Cigarette (EC) aerosols using a combination of advanced technologies. In the gas phase, the particle number concentration (PNC) of EC aerosols was found to be positively correlated with puff duration whereas the PNC and size distribution may vary with different flavors and nicotine strength. In the liquid phase (water or cell culture media), the size of EC nanoparticles appeared to be significantly larger than those in the gas phase, which might be due to aggregation of nanoparticles in the liquid phase. By using in vitro high-throughput cytotoxicity assays, we have demonstrated that EC aerosols significantly decrease intracellular levels of glutathione in NHOKs in a dose-dependent fashion resulting in cytotoxicity. These findings suggest that EC aerosols cause cytotoxicity to oral epithelial cells in vitro, and the underlying molecular mechanisms may be or at least partially due to oxidative stress induced by toxic substances (e.g., nanoparticles and chemicals) present in EC aerosols.Erratum: PLoS One, 2016 Dec 29; 11(12):e0169380. [This corrects the article DOI: 10.1371/journal.pone.0154447.]. [Description provided by NIOSH]
• Subjects:
  Aerosols Electrical Equipment And Supplies Occupational Health Safety Smokers Tobacco Products
• Keywords:
  Cellular Effects Cigarette Smoking Electronic Devices Oral Cavity Oxidative Stress
• ISSN:
  1932-6203
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  11
• Issue:
  5
• NIOSHTIC Number:
  nn:20055745
• Citation:
  PLoS One 2016 May; 11(5):e0154447
• Contact Point Address:
  Shen Hu, School of Dentistry, University of California Los Angeles, Los Angeles, United States of America
• Email:
  shenhu@ucla.edu
• Federal Fiscal Year:
  2016
• Performing Organization:
  University of California Los Angeles
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  PLoS One
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:50c3b96dac0345087156ef67a7c0e9765d986d3478e66d2510f950fc449e91a410b296e63ff1a493035ab3290fc082246c6f82fe48589bb44e401858d0b78290
• Download URL:
  https://stacks.cdc.gov/view/cdc/213526/cdc_213526_DS1.pdf
• File Type:
  [PDF - 1.89 MB ]