Analysis of the Normal and Damaged Inner Ear, Fifth Edition

Details

• Personal Author:
  Bohne BA ; Harding GW
• Description:
  After completing the dissection of the cochlea, the specimen is ready for quantitative anatomical evaluation. The evaluation should be done first on the flat preparations (Fig. la), using the phase contrast microscope. Once evaluation of the flat preparation has been completed, areas which are judged to require more detailed evaluation can then be sectioned (Fig. 1b) on an ultramicrotome and examined by light or transmission electron microscopy (TEM). Although dissection techniques may vary somewhat depending on the species under study (see Bohne, 2009), the analysis techniques are nearly identical for all animals. It is assumed that readers of this manual have some knowledge of biology, in particular, histological techniques and the histological appearance of cells and basic tissue types. If not, the reader should refer to an introductory histology textbook. This manual will describe the techniques used to: determine the length of the organ of Corti (OC), calculate the percentage of missing sensory cells, evaluate other pathological changes in the cochlear duct and prepare blocks of cochlear duct for semi- thick (1-2.5 um) and thin sectioning. [Description provided by NIOSH]
• Subjects:
  Ear Histology Laboratories Microbiology Occupational Health Pathology Safety Sense Organs
• Keywords:
  Auditory System Ears Laboratory Techniques Microscopic Analysis
• Publisher:
  Washington University School of Medicine
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Handbooks
• Place as Subject:
  Missouri ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-51
• NIOSHTIC Number:
  nn:20055524
• Citation:
  St. Louis, MO: Washington University School of Medicine, 2009 Jun; :1-51
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Manufacturing ; Wholesale and Retail Trade
• Performing Organization:
  Washington University, St. Louis
• Peer Reviewed:
  False
• Start Date:
  20000401
• Source Full Name:
  Analysis of the normal and damaged inner ear, fifth edition
• End Date:
  20130914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b84306ff104bff8f33f0d332d4029aeeb09371a7cccbbf757c350dee0444d75dfbea14a7dfc9a220fa484de5a428f7deab8bbe74cb5d6e91f1e57d414b317e5a
• Download URL:
  https://stacks.cdc.gov/view/cdc/213359/cdc_213359_DS1.pdf
• File Type:
  [PDF - 29.65 MB ]