Details:

Alternative Title:
G3 (Bethesda)
Publisher's site:
http://dx.doi.org/10.1534/g3.113.008250
Personal Author:
Pohl, Thomas J. ; Kolor, Katherine ; Fangman, Walton L. ; Brewer, Bonita J. ; Raghuraman, M. K. ;
Pohl, Thomas J. ; Kolor, Katherine ; Fangman, Walton L. ; Brewer, Bonita J. ; Raghuraman, M. K. ; Less -
Description:
Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time.
Subject:
[+]
ARS
Base Sequence
Binding Sites
Centromere
DNA
DNA-Binding Proteins
DNA Mutational Analysis
DNA Replication
Electrophoresis, Gel, Two-Dimensional
Forkhead Transcription Factors
Genome, Fungal
Investigations
Molecular Sequence Data
Mutagenesis
Origins Of Replication
Replication Origin
Saccharomyces Cerevisiae
Saccharomyces Cerevisiae Proteins
Temporal Regulation
Transcription Factors

Source:
G3 (Bethesda). 2013; 3(11):1955-1963.
Document Type:
Journal Article ;
Funding:
GM 18926/GM/NIGMS NIH HHS/United States ; R01 GM018926/GM/NIGMS NIH HHS/United States ;
GM 18926/GM/NIGMS NIH HHS/United States ; R01 GM018926/GM/NIGMS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f16a8f8c6c328b0594fd2470abdb3a26a0610527433aaf6da61a4aed71961899
File Type:

Supporting Files

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