A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae

Pohl, Thomas J.; Kolor, Katherine; Fangman, Walton L.; Brewer, Bonita J.; Raghuraman, M. K.

doi:10.1534/g3.113.008250

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A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae

Nov 06 2013
By Pohl, Thomas J. ; Kolor, Katherine ; Fangman, Walton L. ; ...
http://dx.doi.org/10.1534/g3.113.008250
Source: G3 (Bethesda). 2013; 3(11):1955-1963.

[PDF-1.45 MB]

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Details:

Alternative Title:

G3 (Bethesda)
Personal Author:

Pohl, Thomas J. ; Kolor, Katherine ; Fangman, Walton L. ; Brewer, Bonita J. ; Raghuraman, M. K.

Pohl, Thomas J. ; Kolor, Katherine ; Fangman, Walton L. ; Brewer, Bonita J. ; Raghuraman, M. K. Less -
Description:

Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time.
Subjects:

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ARS Base Sequence Binding Sites Centromere DNA DNA-Binding Proteins DNA Mutational Analysis DNA Replication Electrophoresis, Gel, Two-Dimensional Forkhead Transcription Factors Genome, Fungal Investigations Molecular Sequence Data Mutagenesis Origins Of Replication Replication Origin Saccharomyces Cerevisiae Saccharomyces Cerevisiae Proteins Temporal Regulation Transcription Factors
Source:

G3 (Bethesda). 2013; 3(11):1955-1963.
Document Type:

Journal Article
Funding:

GM 18926/GM/NIGMS NIH HHS/United States ; R01 GM018926/GM/NIGMS NIH HHS/United States

GM 18926/GM/NIGMS NIH HHS/United States ; R01 GM018926/GM/NIGMS NIH HHS/United States Less -
Collection(s):

CDC Public Access
Main Document Checksum:

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urn:sha256:f16a8f8c6c328b0594fd2470abdb3a26a0610527433aaf6da61a4aed71961899
Download URL:

https://stacks.cdc.gov/view/cdc/21323/cdc_21323_DS1.pdf
File Type:

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