A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae

Details

• Alternative Title:
  G3 (Bethesda)
• Personal Author:
  Pohl, Thomas J. ; Kolor, Katherine ; Fangman, Walton L. ; Brewer, Bonita J. ; Raghuraman, M. K.
• Description:
  Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time.
• Subjects:
  ARS Base Sequence Binding Sites Centromere DNA DNA-Binding Proteins DNA Mutational Analysis DNA Replication Electrophoresis, Gel, Two-Dimensional Forkhead Transcription Factors Genome, Fungal Investigations Molecular Sequence Data Mutagenesis Origins Of Replication Replication Origin Saccharomyces Cerevisiae Saccharomyces Cerevisiae Proteins Temporal Regulation Transcription Factors

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• Source:
  G3 (Bethesda). 2013; 3(11):1955-1963.
• Document Type:
  Journal Article
• Funding:
  GM 18926/GM/NIGMS NIH HHS/United States ; R01 GM018926/GM/NIGMS NIH HHS/United States
• Volume:
  3
• Issue:
  11
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f16a8f8c6c328b0594fd2470abdb3a26a0610527433aaf6da61a4aed71961899
• Download URL:
  https://stacks.cdc.gov/view/cdc/21323/cdc_21323_DS1.pdf
• File Type:
  [PDF - 1.45 MB ]