A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae
Published Date:Nov 06 2013
Source:G3 (Bethesda). 2013; 3(11):1955-1963.
DNA Mutational Analysis
Electrophoresis, Gel, Two-Dimensional
Forkhead Transcription Factors
Molecular Sequence Data
Origins Of Replication
Saccharomyces Cerevisiae Proteins
Funding:GM 18926/GM/NIGMS NIH HHS/United States
R01 GM018926/GM/NIGMS NIH HHS/United States
Description:Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time.
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