Influence of Impurities from Manufacturing Process on the Toxicity Profile of Boron Nitride Nanotubes

Details

• Personal Author:
  Bowers L ; Erdely A ; Eye T ; Friend S ; Hubczak J ; Jakubinek M ; Kim KS ; Kodali V ; Leonard, Stephen S. ; Roberts, Jennifer R. ; Stefaniak, Aleksandr B. ; Wolfarth MG ; Xin X
• Description:
  The toxicity of boron nitride nanotubes (BNNTs) has been the subject of conflicting reports, likely due to differences in the residuals and impurities that can make up to 30-60% of the material produced based on the manufacturing processes and purification employed. Four BNNTs manufactured by induction thermal plasma process with a gradient of BNNT purity levels achieved through sequential gas purification, water and solvent washing, allowed assessing the influence of these residuals/impurities on the toxicity profile of BNNTs. Extensive characterization including infrared and X-ray spectroscopy, thermogravimetric analysis, size, charge, surface area, and density captured the alteration in physicochemical properties as the material went through sequential purification. The material from each step is screened using acellular and in vitro assays for evaluating general toxicity, mechanisms of toxicity, and macrophage function. As the material increased in purity, there are more high-aspect-ratio particulates and a corresponding distinct increase in cytotoxicity, nuclear factor-kB transcription, and inflammasome activation. There is no alteration in macrophage function after BNNT exposure with all purity grades. The cytotoxicity and mechanism of screening clustered with the purity grade of BNNTs, illustrating that greater purity of BNNT corresponds to greater toxicity. [Description provided by NIOSH]
• Subjects:
  Biological Assay Immune System Manufacturing And Industrial Facilities Occupational Health Safety Spectrum Analysis Toxicology
• Keywords:
  Aspect Ratio Author Keywords: NALP3 Inflammasome Bioassays Boron Nitride Boron Nitride Nanotubes Gravimetric Analysis Immune Reaction Infrared Spectroscopy Manufacturing Mechanism-based Screening Nanotoxicology Nanotubes Nuclear Factor-kappaB Physiological Chemistry Purity Residuals Spectrographic Analysis Toxicity X-rays

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• ISSN:
  1613-6810
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  18
• Issue:
  52
• NIOSHTIC Number:
  nn:20066372
• Citation:
  Small 2022 Dec; 18(52):2203259
• Contact Point Address:
  Aaron Erdely, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
• Email:
  efi4@cdc.gov
• CAS Registry Number:
  Boron nitride (CAS RN 10043-11-5)
• Federal Fiscal Year:
  2023
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Small
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1ef1dc4ea34a81e332c4ceb03a97f16d0e431edc2bc51aadb0fe35b626c099909b0fe776fa9c2795901070990d8462c5839f2aa9464f6564aec54a854bb0050b
• Download URL:
  https://stacks.cdc.gov/view/cdc/212835/cdc_212835_DS1.pdf
• File Type:
  [PDF - 2.27 MB ]