Airborne Fungal and Bacterial Microbiome in Classrooms of Elementary Schools During the COVID-19 Pandemic Period: Effects of School Disinfection and Other Environmental Factors

Details

• Personal Author:
  Lee BG ; Park, Ju-Hyeong ; Yang JIL ; Yeo M-K
• Description:
  The aim of the study was to examine the effects of environmental factors including disinfection on airborne microbiome during the coronavirus disease 2019 pandemic, we evaluated indoor and outdoor air collected from 19 classrooms regularly disinfected. Extracted bacterial and fungal DNA samples were sequenced using the Illumina MiSeq(TM) platform. Using bacterial DNA copy number concentrations from qPCR analysis, multiple linear regressions including environmental factors as predictors were performed. Microbial diversity and community composition were evaluated. Classrooms disinfected with spray ≤1 week before sampling had lower bacterial DNA concentration (3116 DNA copies/m3) than those >1 week (5003 copies/m3) (p-values = 0.06). The bacterial DNA copy number concentration increased with temperature and was higher in classrooms in coastal than inland cities (p-values <0.01). Bacterial diversity in outdoor air was higher in coastal than inland cities while outdoor fungal diversity was higher in inland than coastal cities. These outdoor microbiomes affected classroom microbial diversity but bacterial community composition at the genus level in occupied classrooms were similar between coastal and inland cities. Our findings emphasize that environmental conditions including disinfection, climate, and school location are important factors in shaping classroom microbiota. Yet, further research is needed to understand the effects of modified microbiome by disinfection on occupants' health. [Description provided by NIOSH]
• Subjects:
  Air Pollution, Indoor Communicable Diseases Disinfectants Fungi Occupational Health Safety
• Keywords:
  Author Keywords: Bioaerosol Bacteria Bioaerosols Climatic Factors COVID-19 Disease Prevention Disinfection IAQ Indoor Air Quality Indoor Environments Microbial Test Systems Microbiome NGS PM QPCR

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• ISSN:
  0905-6947
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  32
• Issue:
  9
• NIOSHTIC Number:
  nn:20066122
• Citation:
  Indoor Air 2022 Sep; 32(9):e13107
• Contact Point Address:
  Ju-Hyeong Park, Respiratory Health Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505, USA
• Email:
  gzp8@cdc.gov
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Services
• Peer Reviewed:
  True
• Source Full Name:
  Indoor Air
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9a240cd9a467745dbde5c727f5eaeb9012af52915e4ef5f6b485b77f0a110f17e35ce8a81f0bcca3862961f5240ba7e9866c5338ea3435175729751be413914a
• Download URL:
  https://stacks.cdc.gov/view/cdc/212635/cdc_212635_DS1.pdf
• File Type:
  [PDF - 1.12 MB ]