A Cross-Sectional Study of Staphylococcus aureus Colonization and the Nasal and Oropharyngeal Microbiomes

Details

• Personal Author:
  Dalman M ; Kates AE ; Smith TC ; Torner JC
• Description:
  Background: Staphylococcus aureus is a frequent cause of both infections globally. Colonization with the organism is known to increase the risk of developing infections and occurs in roughly one third of the general population. While many factors influence colonization, it has been demonstrated other members of the microbiome influence colonization with S. aureus. Here, we assessed the nasal and oropharyngeal microbiomes of healthy participants in relation to S. aureus colonization in a cross-sectional study using 16s rRNA sequencing of the v1-v3 region. As livestock workers have also been shown to be at an increased risk of carriage, we have also assessed microbiota differences in colonization status in a population of livestock workers. Results: In both the nares and oropharynx, there were no microbiota differentially abundant between colonized and non-colonized persons. However, there was a significant difference in the beta diversity (Bray-Curtis distances) between carriers and non-carriers (P=0.002). When considering carriage stratified by livestock exposure, there were a number of differences. Most notably, colonized livestock workers had significantly more Porphyomonas (2-fold change = -8.54, P = 0.03) than the non-colonized livestock workers. Conclusions: S. aureus is a frequent colonizer of the human upper respiratory tract, including the nares and oropharynx and causes a wide range of infections. Livestock workers are at increased risk for carriage. Interventions such as improving oral hygiene may lead to decreased S. aureus carriage by reducing other bacterial species such as Porphyomonas. Larger, longitudinal studies are needed to better explore what microorganisms may be associated with S. aureus colonization. [Description provided by NIOSH]
• Subjects:
  Agriculture Animals Livestock Microbiology Nose Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Sense Organs

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• Keywords:
  16s RRNA Agricultural Workers Anaerobic Bacteria Animal Handlers Animal Husbandry Workers Author Keywords: Staphylococcus Aureus Bacteria Bacterial Infections Colonization Cross Sectional Studies Deoxyribonucleic Acids DNA Epidemiology Humans Infectious Agents Intervention Microbiome Microorganisms Nasal Cavity Oral Cavity Oropharynx Pathogenicity Respiratory Infections Respiratory System Disorders Ribonucleic Acids Risk Analysis Risk Factors RNA Staphylococcus Aureus Staphylococcus Aureus Infection Throat

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• ISSN:
  1745-6673
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Iowa ; Ohio ; OSHA Region 5 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20052375
• Citation:
  J Occup Med Toxicol 2017 Jun; :[Preprint]
• Contact Point Address:
  Tara C. Smith, PhD, Department of Biostatistics, Epidemiology and Environmental Health Services, College of Public Health, Kent State University, 750 Lowry Hall, Kent, OH 44240
• Email:
  tsmit176@kent.edu
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Iowa, Iowa City
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Occupational Medicine and Toxicology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8317287abb804d6e103a37f4ae9bd11f5616da575624201ceef7024ba66164a8e0fffc048cbcfa3889666efa16fe6fe377130edb53c6020183a462b48a600663
• Download URL:
  https://stacks.cdc.gov/view/cdc/211908/cdc_211908_DS1.pdf
• File Type:
  [PDF - 2.07 MB ]