Kinetics of miR-122 Expression in the Liver during Acute HCV Infection

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Choi, Youkyung ; Dienes, Hans-Peter ; Krawczynski, Kris
• Description:
  The relationships among micro RNA-122 (miR-122) expression in the liver, hepatitis C virus (HCV) replication and hepatic damage were analyzed in three chimpanzees observed for 180 days after inoculation with HCV genotype 1a. Levels of miR-122 in the liver and serum were measured by real-time RT PCR in serial liver biopsies and serum samples. Hepatic miR-122 levels were normalized separately for each of three chimpanzees with small RNAs and microRNAs that are endogenous to the liver and are stably expressed. Two- to 4-fold rise in hepatic miR-122 levels was observed at the onset of HCV infection (the first 4 weeks) when HCV titers in the liver and serum increased rapidly in all three chimpanzees in concordance with in vitro data indicating the miR-122 significance for HCV replication. Between 10 to 14 weeks after inoculation, when hepatic and serum HCV RNA titers exceeded 3 logs and alanine aminotransferase (ALT) activity was elevated, hepatic miR-122 levels were in decline. Cumulative data derived from all three chimpanzees from 180 days of observation documented an inverse (negative) correlation between hepatic miR-122 and HCV RNA in the liver and serum and positive correlation between level of serum miR-122 and HCV replication. Subsequent rise of miR-122 level during HCV clearance and ALT normalization suggested a tri-phasic occurrence of the relationship among hepatic miR-122 expression, HCV replication and hepatic destruction, which was the most apparent in one chimpanzee but less evident in two other animals. In vivo kinetics of hepatic and serum miR-122, HCV replication and hepatic destruction reflects complexities of the virus-host interaction during the acute phase of HCV infection.
• Subjects:
  Animals Caenorhabditis Elegans Gene Expression Regulation Hepacivirus Hepatitis C Interferon Type I Kinetics Liver MicroRNAs Pan Troglodytes Viral Load

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• Source:
  PLoS One. 2013; 8(10).
• Document Type:
  Journal Article
• Volume:
  8
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:952a31da4dc26f0a771acfff6cea1241ff48d75a38a5beb0f9e8c96a6827f3fe
• Download URL:
  https://stacks.cdc.gov/view/cdc/21181/cdc_21181_DS1.pdf
• File Type:
  [PDF - 779.25 KB ]