Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru

Akinyi, Sheila; Hayden, Tonya; Gamboa, Dionicia; Torres, Katherine; Bendezu, Jorge; Abdallah, Joseph F.; Griffing, Sean M.; Quezada, Wilmer Marquiño; Arrospide, Nancy; De Oliveira, Alexandre Macedo; Lucas, Carmen; Magill, Alan J.; Bacon, David J.; Barnwell, John W.; Udhayakumar, Venkatachalam

doi:10.1038/srep02797

i

Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru

Supporting Files

Sep 30 2013
By Akinyi, Sheila ; Hayden, Tonya ; Gamboa, Dionicia ; ...

Details

Alternative Title:

Sci Rep
Personal Author:

Akinyi, Sheila ; Hayden, Tonya ; Gamboa, Dionicia ; Torres, Katherine ; Bendezu, Jorge ; Abdallah, Joseph F. ; Griffing, Sean M. ; Quezada, Wilmer Marquiño ; Arrospide, Nancy ; De Oliveira, Alexandre Macedo ; Lucas, Carmen ; Magill, Alan J. ; Bacon, David J. ; Barnwell, John W. ; Udhayakumar, Venkatachalam
Description:

The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.
Subjects:

Animals Antigens, Protozoan Article Bayes Theorem Cluster Analysis Gene Deletion Haplotypes Humans Microsatellite Repeats Parasites Peru Phenotype Plasmodium Falciparum Prevalence Protozoan Proteins
Source:

Sci Rep. 2013; 3.
Document Type:

Journal Article
Funding:

D43TW007120/TW/FIC NIH HHS/United States ; U19AI089681/AI/NIAID NIH HHS/United States
Volume:

3
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:96d5cd38ee567a0edfe333ed4142745bd52cd4d3e929d5f29b6bf937da2492de
Download URL:

https://stacks.cdc.gov/view/cdc/21169/cdc_21169_DS1.pdf
File Type:

[PDF - 715.71 KB ]

ON THIS PAGE

Details Supporting Files

CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.