Screening In Vitro Toxicity Endpoints of Carbon Nanotubes and Nanofibers from United States Facilities

Details

• Personal Author:
  Birch EM ; Dahm, Matthew M. ; Erdely AD ; Evans DE ; Eye T ; Kodali VK ; Schubauer-Berigan, Mary K. ; Shoeb M ; Smith K ; Stefaniak, Aleksandr B. ; Stueckle, Todd A. ; Yanamala N ; Birch EM ; Dahm, Matthew M. ; Erdely AD ; Evans DE ; Eye T ; Kodali VK ; Schubauer-Berigan, Mary K. ; Shoeb M ; Smith K ; Stefaniak, Aleksandr B. ; Stueckle, Todd A. ; Yanamala N
• Description:
  Significant investment has been made in recent years to harness the economic potential of utilizing the unique characteristics of carbon nanotubes and nanofibers (CNT/F) to advance material science applications. Unfortunately, those same characteristics that provide significant potential for application may also confer adverse health effects. CNT/F represent a broad class of materials and we hypothesized that not all CNT/F produced or utilized in US facilities confer similar toxicities. Extensive characterization was done for seven different multiwalled CNT and two CNF that were selected based on primary particle diameter ranging from 10 to 150 nm. The specific surface areas ranged from 18 to 238 m2/g and decreased with increasing primary particle diameter. Key molecular-initiating events (MIE) and functional responses were screened over a wide dose range (0-60 microg/ml) in a human monocytic cell line (THP-1), both wild-type and NLRP3 inflammasome deficient cells, and in primary human lung fibroblast cells (PHF). Membrane damage and cell proliferation in THP-1 challenged with CNT/F for 24 h segregated by diameter with materials greater than or equal to 50 nm in diameter inducing greater toxicity. There was approximately 150 fold change in IL-1beta secreted in THP-1 WT vs NLRP3 deficient cells and for all the CNT/F tested, apart from having a dose-dependent increase, the level of secretions was enhanced with increasing primary particle diameter. Similarly, CNT/F exposure (0-2 microg/cm2) to PHFs elicited increased cell proliferation and collagen I production that was greater with increased primary particle diameter. Structure activity correlations to date indicate that CNT/F could be broadly classified into two groups, materials below 50 nm in primary particle diameter were less bioactive/toxic than tubes greater than 50 nm. Ongoing research and modeling will further elucidate relationships between physicochemical characteristics and toxicity profile of various CNT/F. [Description provided by NIOSH]
• Subjects:
  Lung Occupational Health Respiratory System Safety Toxicology
• Keywords:
  Carbon Nanofibers Carbon Nanotubes Cell Proliferation Cell Type CNF Dose Response Fibroblasts Health Effects In Vitro Study Lung Cells Membrane Dysfunction Multi-walled Carbon Nanotubes MWCNT Nanotechnology Nanotoxicology Particle Diameter Toxic Effects

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology ; RHD - Respiratory Health Division ; DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies ; HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology ; RHD - Respiratory Health Division ; DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  162
• Issue:
  1
• NIOSHTIC Number:
  nn:20051242
• Citation:
  Toxicologist 2018 Mar; 162(1):402
• CAS Registry Number:
  Carbon nanotubes (CAS RN 308068-56-6) ; Carbon nanotubes (CAS RN 308068-56-6)
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Manufacturing ; Healthcare and Social Assistance ; Services ; Manufacturing ; Healthcare and Social Assistance ; Services
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 57th Annual Meeting and ToxExpo, March 11-15, 2018, San Antonio, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6485ad13376684a4ab6e628cfc78cf1c5ae5becf9f1ef6ee31319d44f6438b030610962722c2be6aefe4193960b2fa5f12d0c47358b14b86301ff568ddd86b39
• Download URL:
  https://stacks.cdc.gov/view/cdc/211308/cdc_211308_DS1.pdf
• File Type:
  [PDF - 857.12 KB ]