Short Term Dynamics of the Sputum Microbiome Among COPD Patients

Details

• Personal Author:
  Clarke JL ; Heires AJ ; LeVan TD ; Romberger DJ ; Sinha R ; Smith LM ; Weissenburger-Moser LA
• Description:
  Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder characterized by incompletely reversible airflow obstruction. The complexity of the lung microbial community in COPD patients has been highlighted in recent years. Evidence suggests that transplantation, medications, age, and disease severity influence microbial community membership. However, the dynamics of the lung microbiome in stable COPD patients remain poorly understood. In this study, we completed a longitudinal 16S ribosomal RNA survey of the lung microbiome on replicate sputum samples collected from 4 former smokers with COPD (Stage 2) within a 2-day time period. Samples from each individual over the two-day period were similar based on alpha-diversity, principle component analysis and taxonomy at the phyla and genera level. Sputum samples from COPD patients were also collected between 2-9 months of follow-up. Data suggest an increased variability of the sputum microbiota when comparing samples collected ≤ 3 months compared to those collected >/= 4 months; however, no statistically significant shifts in the abundance (>2-fold) of taxa between the two time points was observed. Bacterial composition and the number of operational taxonomic units (OTUs) remained similar over time. Results from this study suggest that the sputum microbiome is relatively stable in clinically stable COPD patients (Stage 2). This study furthers our understanding of the dynamics of the lung microbiome in COPD patients. [Description provided by NIOSH]
• Subjects:
  Occupational Health Respiratory System Respiratory Tract Diseases Safety Smokers
• Keywords:
  Chronic Obstructive Pulmonary Disease COPD Respiratory Diseases Respiratory System Disorders Smoking
• ISSN:
  1932-6203
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  3
• NIOSHTIC Number:
  nn:20051231
• Citation:
  PLoS One 2018 Mar; 13(3):e0191499
• Contact Point Address:
  Tricia D. LeVan, University of Nebraska, Department of Food Science & Technology, Lincoln, NE, United States of America
• Email:
  tlevan@unmc.edu
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center - Omaha
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  PLoS One
• End Date:
  20270831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d7640cb2761486a61691655c9ffe0605560061e0520836ab6c4ea5e1102ffec5934aaecd8c2c855145577214b22c577ed0a87aa8da0ea28011abc3d57d75cfe9
• Download URL:
  https://stacks.cdc.gov/view/cdc/211304/cdc_211304_DS1.pdf
• File Type:
  [PDF - 3.39 MB ]