Characterization of Pulmonary Responses in Mice to Asbestos/Asbestiform Fibers Using Gene Expression Profiles

Details

• Personal Author:
  Gutkin DW ; Kisin ER ; Shurin MR ; Shvedova AA ; Yanamala N
• Description:
  Humans exposed to asbestos and/or asbestiform fibers are at high risk of developing many lung diseases, including asbestosis, lung cancer, and malignant mesothelioma. However, the disease-causing potential and related processes associated with various asbestos/asbestiform fiber exposures in triggering the different (non-)carcinogenic outcomes is still largely unknown. In this study, we investigated whether exposure to different asbestos/asbestiform fibers leads to differences in inflammatory responses and gene expression profiles at acute/subacute phases that can be related to pathological outcomes observed at extended time points of post-exposure. We exposed mice to asbestos (crocidolite, tremolite asbestos), asbestiform fibers (erionite), and a low-pathogenicity mineral fiber (wollastonite) using oropharyngeal aspiration. We observed some shared inflammatory and tissue damage responses, albeit to different extents, at day 1 and 7 post-exposure. In addition, exposure to different fibers also exhibited distinct changes in the regulation and release of a number of inflammatory mediators. Further, a detailed comparison of gene regulation changes in the lungs on day 7 post-exposure also suggested differential biological responses that were consistent with histopathological changes at day 7 and 56 post-exposure. Taken together, these results suggest clear differences in the magnitude of various pulmonary responses and gene regulation that were consistent with pathological alterations upon exposure to the four asbestos/asbestiform fibers studied. Further mechanistic studies focusing on long-term endpoints are critical for understanding how early biological responses are associated with pulmonary exposures to asbestos/asbestiform materials and their ability in triggering different carcinogenic outcomes, i.e., lung cancer versus mesothelioma. [Description provided by NIOSH]
• Subjects:
  Asbestos Immune System Inorganic Chemicals Laboratories Lung Diseases Occupational Health Respiratory Tract Diseases Safety Toxicology
• Keywords:
  Asbestos Fibers Asbestosis Cytokines Gene Expression Histopathology Immune Reaction Laboratory Animals Lung Disease Tissue Culture
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 6 ; Pennsylvania ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  44
• Volume:
  162
• Issue:
  1
• NIOSHTIC Number:
  nn:20051140
• Citation:
  Toxicologist 2018 Mar; 162(1):44
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4)
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Mining
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 57th Annual Meeting and ToxExpo, March 11-15, 2018, San Antonio, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:69c8169c4e208742ee4452f979c0e77d90a183e40a5ef1de5b223be40d202eb8968daafcbd09071989c4a74671df4c9e232ebd6da72b65177cf9ddf056a6a1dd
• Download URL:
  https://stacks.cdc.gov/view/cdc/211248/cdc_211248_DS1.pdf
• File Type:
  [PDF - 832.70 KB ]