microRNA in Experimental Models of Chemical Sensitization

Details

• Personal Author:
  Anderson, Stacey E.
• Description:
  Allergic disease is an important occupational health concern, with work-related asthma and allergic contact dermatitis being the most frequently diagnosed occupational illnesses. Understanding the mechanisms behind allergic disease is critical for treatment and prevention. The regulatory potential of microRNAs (miRNAs) has been recognized in a variety of disease states, including allergic disease; however, the roles of miRNAs in chemical sensitization are largely unknown. Increased expression of multiple miRNAs during toluene 2,4-diisocyanate (TDI) sensitization was observed, and several putative mRNA targets identified for these miRNAs were directly related to regulatory T-cell (Treg) differentiation and function, including Foxp3 and Runx3. Specifically, miR-210 expression increased in the mouse draining lymph node (dLN) and Treg subsets following dermal TDI sensitization. Alterations in dLN mRNA and protein expression of Treg-related genes/putative miR-210 targets (foxp3, runx3, ctla4, and cd25) were observed at multiple time points following TDI exposure and in ex vivo systems. A Treg suppression assay, including a miR-210 mimic, was utilized to investigate the suppressive ability of Tregs. Cells derived from TDI-sensitized mice treated with miR-210 mimic had less expression of miR-210 compared to the acetone control, suggesting other factors, such as additional miRNAs, might be involved in the regulation of the functional capabilities of these cells. These novel findings indicate that miR-210 may have an inhibitory role in Treg function during TDI sensitization. Because the functional roles of miRNAs have not been previously elucidated in a model of chemical sensitization, these data contribute to the understanding of the potential immunologic mechanisms of chemical-induced allergic disease. [Description provided by NIOSH]
• Subjects:
  Acetone Animals Asthma Asthma, Occupational Cells, Cultured Chemistry Genetics Hypersensitivity Immune System Isocyanates Laboratories Lymphatic System Occupational Health Respiratory System Respiratory Tract Diseases Safety Skin Toluene Toxicology

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• Keywords:
  Acetones Allergic Disorders Analytical Models Bronchial Asthma Cell Alteration Cell Cultures Cell Differentiation Cell Function Cellular Function Chemical Composition Chemical Properties Chemicals Dermal Exposures Diisocyanates Gene Expression Gene Regulation Genes Immune System Immunoassays Laboratory Animals Lymph Nodes Models Molecular Structure Proteins Ribonucleic Acids RNA Sensitization Sensitizers Toluene-2,4-diisocyanate

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  7
• Volume:
  162
• Issue:
  1
• NIOSHTIC Number:
  nn:20051133
• Citation:
  Toxicologist 2018 Mar; 162(1):7
• CAS Registry Number:
  Acetone (CAS RN 67-64-1) ; Toluene 2,4-diisocyanate (CAS RN 584-84-9) ; Toluene diisocyanate (CAS RN 26471-62-5)
• Federal Fiscal Year:
  2018
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 57th Annual Meeting and ToxExpo, March 11-15, 2018, San Antonio, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1b22c22cfbab6106f247ccc8ee682a8853e83839455391ce5969189cc1a5e9bc608ab456acb81ebda6f95b397450625972b7b1817fe3c7e5739af29dc6b9d3a9
• Download URL:
  https://stacks.cdc.gov/view/cdc/211243/cdc_211243_DS1.pdf
• File Type:
  [PDF - 832.45 KB ]