Inhalation Exposure to Three-Dimensional Printer Emissions Stimulates Acute Hypertension and Microvascular Dysfunction

Details

• Personal Author:
  Abukabda AB ; Duling, Matthew G. ; LeBouf, Ryan F. ; McBride CR ; Nurkiewicz TR ; Stefaniak, Aleksandr B. ; Yi J
• Description:
  Fused deposition modeling [FDM(TM)], or three-dimensional (3D) printing has become routine in industrial, occupational and domestic environments. We have recently reported that 3D printing emissions (3DPE) are complex mixtures, with a large ultrafine particulate matter component. Additionally, we and others have reported that inhalation of xenobiotic particles in this size range is associated with an array of cardiovascular dysfunctions. Sprague-Dawley rats were exposed to 3DPE aerosols via nose-only exposure for approximately 3 h. Twenty-four hours later, intravital microscopy was performed to assess microvascular function in the spinotrapezius muscle. Endothelium-dependent and -independent arteriolar dilation were stimulated by local microiontophoresis of acetylcholine (ACh) and sodium nitroprusside (SNP). At the time of experiments, animals exposed to 3DPE inhalation presented with a mean arterial pressure of 125 +/- 4 mm Hg, and this was significantly higher than that for the sham-control group (94 +/- 3 mm Hg). Consistent with this pressor response in the 3DPE group, was an elevation of approximately 12% in resting arteriolar tone. Endothelium-dependent arteriolar dilation was significantly impaired after 3DPE inhalation across all iontophoretic ejection currents (0-27 +/- 15%, compared to shamcontrol: 15-120 +/- 21%). Endothelium-independent dilation was not affected by 3DPE inhalation. These alterations in peripheral microvascular resistance and reactivity are consistent with elevations in arterial pressure that follow 3DPE inhalation. Future studies must identify the specific toxicants generated by FDM(TM) that drive this acute pressor response. [Description provided by NIOSH]
• Subjects:
  Aerosols Cardiovascular Diseases Cardiovascular System Laboratories Musculoskeletal System Occupational Health Printing, Three-Dimensional Respiratory System Safety Toxicology
• Keywords:
  3D Printing Aerosol Particles Author Keywords: Ultrafine Particle Endothelium Hypertension Inhalation Laboratory Animals Microcirculation Muscle Tissue Printer-emitted Particles Three Dimensional Printing Ultrafine Particulates

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• ISSN:
  0041-008X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-5
• Volume:
  335
• NIOSHTIC Number:
  nn:20050465
• Citation:
  Toxicol Appl Pharmacol 2017 Nov; 335:1-5
• Contact Point Address:
  T. R. Nurkiewicza, Department of Physiology, Pharmacology and Neuroscience, 1 Medical Center Drive, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV, 26506-9105, USA
• Email:
  tnurkiewicz@hsc.wvu.edu
• Federal Fiscal Year:
  2018
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Applied Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:40a192892faed3f465a7bea24f7745a4fe18f62d1ec5fd38893ea7ce4f01ca800808361007d907acdc819ed4e7a24f85a19121ed060c0989097388c51170fab5
• Download URL:
  https://stacks.cdc.gov/view/cdc/210792/cdc_210792_DS1.pdf
• File Type:
  [PDF - 260.40 KB ]