Hypomethylation of Dual Specificity Phosphatase 22 Promoter Correlates with Duration of Service in Firefighters and Is Inducible by Low-Dose Benzo[a]pyrene

Details

• Personal Author:
  Baxter CS ; Haynes E ; Ho SM ; Lam HM ; Levin L ; Ouyang B ; Yeramaneni S
• Description:
  OBJECTIVE: Firefighters (FFs) are chronically exposed to smoke and products of incomplete combustion, which frequently contain polycyclic aromatic hydrocarbons (PAHs). This study examined the possibility of an association between PAH-induced epigenetic alterations and occupational firefighting exposure. METHODS: Promoter methylation was analyzed in four genes in blood DNA from 18 FFs and 20 non-FFs (controls). Jurkat and human normal prostate epithelial cells were treated with benzo[a]pyrene to ascertain the epigenetic effects of this type of agent. RESULTS: Firefighters had a higher prevalence of dual specificity phosphatase 22-promoter hypomethylation in blood DNA (P = 0.03) and the extent of hypomethylation correlated with duration of firefighting service (P = 0.04) but not with age. Benzo[a]pyrene reduced promoter methylation and increased gene expression of the same gene in Jurkat and normal prostate epithelial cells. CONCLUSIONS: Cumulative occupational exposure to combustion-derived PAHs during firefighting can cause epigenetic changes in promoters of specific genes. [Description provided by NIOSH]
• Subjects:
  Benzopyrenes Biomarkers Blood Environmental Exposure Firefighters Genetics Hazardous Substances Hematologic Tests Hydrocarbons Occupational Exposure Occupational Health Polycyclic Aromatic Hydrocarbons Risk Assessment Risk Factors Safety

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• Keywords:
  Blood Cells Blood Samples Chronic Exposure Combustible Materials Combustion Deoxyribonucleic Acids DNA Exposure Levels Fire Fighters Genes Genotoxic Effects Polycyclic Aromatic Hydrocarbons Risk Factors Smoke Inhalation Toxic Effects Toxic Materials

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• ISSN:
  1076-2752
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  54
• Issue:
  7
• NIOSHTIC Number:
  nn:20050249
• Citation:
  J Occup Environ Med 2012 Jul; 54(7):774-780
• Contact Point Address:
  Shuk-Mei Ho, PhD, Department of Environmental Health, Kettering Complex, Room 128, 3223 Eden Ave, University of Cincinnati Medical Center, Cincinnati, OH 45267
• Email:
  shuk-mei.ho@uc.edu
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8)
• Federal Fiscal Year:
  2012
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Occupational and Environmental Medicine
• End Date:
  20260630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:602d00fb35ec5e15441c4acc532f0d82d70e9a9889f5f75c6c95231c06e394b82a178b352a0af1e5263afead1845b2f8267ebd2c91ed5b718caad9cf3befa52c
• Download URL:
  https://stacks.cdc.gov/view/cdc/210673/cdc_210673_DS1.pdf
• File Type:
  [PDF - 402.74 KB ]