Continuous Separation of Blood Cells in Spiral Microfluidic Devices

Details

• Personal Author:
  Nivedita N ; Papautsky I
• Description:
  Blood cell sorting is critical to sample preparation for both clinical diagnosis and therapeutic research. The spiral inertial microfluidic devices can achieve label-free, continuous separation of cell mixtures with high throughput and efficiency. The devices utilize hydrodynamic forces acting on cells within laminar flow, coupled with rotational Dean drag due to curvilinear microchannel geometry. Here, we report on optimized Archimedean spiral devices to achieve cell separation in less than 8 cm of downstream focusing length. These improved devices are small in size (<1 in.(2)), exhibit high separation efficiency (approximately 95%), and high throughput with rates up to 1×10(6) cells per minute. These device concepts offer a path towards possible development of a lab-on-chip for point-of-care blood analysis with high efficiency, low cost, and reduced analysis time. [Description provided by NIOSH]
• Subjects:
  Blood Cells, Cultured Diagnosis Hematologic Tests Occupational Health Safety
• Keywords:
  Blood Analysis Blood Cells Blood Samples Blood Tests Cell Biology Cell Cultures Clinical Diagnosis Diagnostic Techniques Diagnostic Tests Physiological Testing Qualitative Analysis Therapeutic Agents

  More +
• ISSN:
  1932-1058
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  7
• Issue:
  5
• NIOSHTIC Number:
  nn:20050155
• Citation:
  Biomicrofluidics 2013 Sep; 7(5):054101
• Contact Point Address:
  Ian Papautsky, BioMicroSystems Lab, School of Electronic and Computing Systems, University of Cincinnati, Cincinnati, Ohio 45221
• Email:
  ian.papautsky@uc.edu
• Federal Fiscal Year:
  2013
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Biomicrofluidics
• End Date:
  20260630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e6ea1d0c382a4fe3f0732891046942b41ff46e523de4622d58cfb01018152e27127e2b76b9b3a8c371bb76cadcdec1ce6b649e25eb28e65e5189a070963bb27a
• Download URL:
  https://stacks.cdc.gov/view/cdc/210629/cdc_210629_DS1.pdf
• File Type:
  [PDF - 2.54 MB ]