Imaging Mass Spectrometry Reveals Loss of Polyunsaturated Cardiolipins in the Cortical Contusion, Hippocampus, and Thalamus After Traumatic Brain Injury

Details

• Personal Author:
  Amoscato AA ; Anthonymuthu T ; Bayir H ; Fink AB ; Kagan VE ; Kochanek PM ; New LA ; Sparvero LJ ; Watkins S
• Description:
  Traumatic brain injury (TBI) leads to changes in ion fluxes, alterations in mitochondrial function, and increased generation of reactive oxygen species, resulting in secondary tissue damage. Mitochondria play important signaling roles in coordination of multiple metabolic platforms in addition to their well-known role in bioenergetics. Mitochondrial signaling strongly depends on cardiolipin (CL), a mitochondria-specific structurally unusual anionic phospholipid containing four fatty acyl chains. While our previous reports indicated that CL is selectively oxidized and presents itself as a target for the redox therapy following TBI, the topography of changes of CL in the injured brain remained to be defined. Here, we present a matrix-assisted laser desorption/ionization imaging study which reports regio-specific changes in CL, in a controlled cortical impact model of TBI in rats. Matrix-assisted laser desorption/ionization imaging revealed that TBI caused early decreases in CL in the contusional cortex, ipsilateral hippocampus, and thalamus with the most highly unsaturated CL species being most susceptible to loss. Phosphatidylinositol was the only other lipid species that exhibited a significant decrease, albeit to a lesser extent than CL. Signals for other lipids remained unchanged. This is the first study evaluating the spatial distribution of CL loss after acute brain injury. We propose that the CL loss may constitute an upstream mechanism for CL-driven signaling in different brain regions as an early response mechanism and may also underlie the bioenergetic changes that occur in hippocampal, cortical, and thalamic mitochondria after TBI. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Lipids Occupational Health Safety
• Keywords:
  Author Keywords: Cardiolipin Bioenergetics Cell Signaling Hippocampus Imaging Mass Spectrometry Imaging Techniques Mass Spectrometry Oxidation Reduction Reactions Reactive Oxygen Species ROS TBI Thalamus Traumatic Brain Injury

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• ISSN:
  0022-3042
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  139
• Issue:
  4
• NIOSHTIC Number:
  nn:20049932
• Citation:
  J Neurochem 2016 Nov; 139(4):659-675
• Contact Point Address:
  Hulya Bayir, MD, Children's Hospital of Pittsburgh, 4401 Penn Avenue, Pittsburgh, PA 15224, USA
• Email:
  bayihx@ccm.upmc.edu
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Neurochemistry
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:83cf6bf62130bf979af8a25601043d4a0d92c6531baaa331cfe9638359d5303f19757eb84cdcf97c3a52bd42c0543cae6091a7f7ade26e27244e1a5c5c02eba1
• Download URL:
  https://stacks.cdc.gov/view/cdc/210520/cdc_210520_DS1.pdf
• File Type:
  [PDF - 5.25 MB ]