Altered Ion Transport in Normal Human Bronchial Epithelial Cells Following Exposure to Chemically Distinct Metal Welding Fume Particles

Details

• Personal Author:
  Antonini JM ; Fedan, Jeffery S. ; Meighan TG ; Thompson JA ; Zeidler-Erdely PC
• Description:
  Welding fume inhalation causes pulmonary toxicity, including susceptibility to infection. We hypothesized that airway epithelial ion transport is a target of fume toxicity, and investigated the effects of fume particulates from manual metal arc-stainless steel (MMA-SS) and gas metal arc-mild steel (GMA-MS) on ion transport in normal human bronchial epithelium (NHBE) cultured in air-interface. MMA-SS particles, more soluble than GMA-MS particles, contain Cr, Ni, Fe and Mn; GMA-MS particles contain Fe and Mn. MMA-SS or GMA-MS particles (0.0167-166.7 ug/cm2) were applied apically to NHBEs. After 18 h transepithelial potential difference (Vt), resistance (Rt), and short circuit current (Isc) were measured. Particle effects on Na+ and Cl¯ channels and the Na+,K+,2Cl¯-cotransporter were evaluated using amiloride (apical), 5-nitro-2-[(3-phenylpropyl)amino]benzoic acid (NPPB, apical), and bumetanide (basolateral), respectively. MMA-SS (0.0167-16.7 ug/cm2) increased basal Vt. Only 16.7 ug/cm2 GMA-MS increased basal Vt significantly. MMA-SS or GMA-MS exposure potentiated Isc responses (decreases) to amiloride and bumetanide, while not affecting those to NPPB, GMA-MS to a lesser degree than MMA-SS. Variable effects on Rt were observed in response to amiloride, and bumetanide. Generally, MMASS was more potent in altering responses to amiloride and bumetanide than GMA-MS. Hyperpolarization occurred in the absence of LDH release, but decreases in Vt, Rt, and Isc at higher fume particulate doses accompanied LDH release, to a greater extent for MMA-SS. Thus, Na+ transport and Na+,K+,2Cl¯-cotransport are affected by Fume exposure; MMA-MS is more potent than GMA-MS. Enhanced Na+ absorption and decreased airway surface liquid could compromise defenses against infection. [Description provided by NIOSH]
• Subjects:
  Lung Metals Occupational Health Particulate Matter Respiratory System Safety Toxicology Welding
• Keywords:
  Air-interface Airway Epithelium Arc Welding Author Keywords: Welding Fume Cell Culture Ion Channels Ion Transport Lactate Dehydrogenase Metal Compounds Particle Aerodynamics Particulates Pulmonary Effects Welding Fumes

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• ISSN:
  0041-008X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-6
• Volume:
  236
• NIOSHTIC Number:
  nn:20049690
• Citation:
  Toxicol Appl Pharmacol 2017 Jul; 326:1-6
• Contact Point Address:
  Jeffrey S. Fedan, Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  jsf2@cdc.gov
• CAS Registry Number:
  Chromium (CAS RN 7440-47-3) ; Iron (CAS RN 7439-89-6) ; Manganese (CAS RN 7439-96-5) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Applied Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5f399c8f877055d833bec90a230cc8fcbe964e7b4882f539b7b268641435a243072b6850767076e8c88a10e4dc7c9b5cf783a61ad257a9269d7d4346e2ec885e
• Download URL:
  https://stacks.cdc.gov/view/cdc/210355/cdc_210355_DS1.pdf
• File Type:
  [PDF - 603.16 KB ]