Measurement of Short-Chain Fatty Acids in Respiratory Samples: Keep Your Assay Above the Water Line

Details

• Personal Author:
  Adar SD ; Curtis JL ; D'Souza J ; Erb-Downward JR ; Evans CR ; Foxman B ; Kachman M ; Kim JH ; Stringer KA ; Yue M
• Description:
  Short-chain fatty acids (SCFAs) are bacterial products that have important biological functions, including maintenance of immune homeostasis. Growing evidence indicates that bacteria residing in the airways of patients with numerous pulmonary diseases as well as in those of healthy individuals are capable of making SCFAs. Therefore, there is growing interest in measuring respiratory SCFA concentrations because they could provide insight into biological processes in the lungs. Sampling the lungs is challenging; the most common biospecimen is BAL fluid, which requires bronchoscopy under sedation. This invasive procedure is labor intensive and costly, and it may not be feasible in unstable critically ill patients. Exhaled breath condensate (EBC) is an easily acquired, abundant biofluid that could be used as an alternative to BAL. To test the utility of EBC for this purpose, we measured SCFAs and 16s ribosomal (r)RNA in paired BAL and EBC samples acquired from healthy control subjects. [Description provided by NIOSH]
• Subjects:
  Biological Assay Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Bacteria Bioassays Biological Materials Fatty Acids Laboratory Techniques Lung Cells
• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  202
• Issue:
  4
• NIOSHTIC Number:
  nn:20070893
• Citation:
  Am J Respir Crit Care Med 2024 Feb; 209(3):334-337
• Email:
  stringek@umich.edu
• Federal Fiscal Year:
  2020
• Performing Organization:
  University of Michigan, Ann Arbor
• Peer Reviewed:
  False
• Start Date:
  20050701
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b285060d3fec9df7db496c501041c53f38a7f8c7afb7d406d7b037238889104c27675cfa9ae7c744ee3cd8dff4e2bf1f537de5365a358b594171a84a7ae62cec
• Download URL:
  https://stacks.cdc.gov/view/cdc/209017/cdc_209017_DS1.pdf
• File Type:
  [PDF - 597.18 KB ]