The Trichloroethylene Metabolite S-(1,2-Dichlorovinyl)-L-Cysteine Inhibits Lipopolysaccharide-Induced Inflammation Transcriptomic Pathways and Cytokine Secretion in a Macrophage Cell Model

Details

• Personal Author:
  Bakulski KM ; Boldenow E ; Dou J ; Harlow O ; Harris SM ; Houskamp E ; Loch-Caruso R ; Scheeres EC ; Schellenboom E
• Description:
  Studies have shown that the trichloroethylene metabolite S-(1,2-dichlorovinyl)-l-cysteine (DCVC) inhibits cytokine secretion in pathogen stimulated fetal membrane tissue but little is known about the mechanism for these effects, including which cell types or transcriptomic pathways are impacted. Macrophages play a critical role in fetal membrane immune responses during infection. We tested the hypothesis that DCVC inhibits lipopolysaccharide (LPS) stimulated inflammation pathways in macrophage-like THP-1 cells. We treated THP-1 cells for 24 h then treated with 1, 5, or 10 µM DCVC for 24 h. After a 4 h incubation with lipopolysaccharide (LPS), we collected RNA and cell media. We performed transcriptomic analysis using RNA sequencing for 5 µM DCVC treatments and quantified cytokine release (IL-1 β, IL-6, and TNF-a) for 1, 5 and 10 µM DCVC treatments. RNA sequencing analysis revealed 1399 differentially expressed genes (FDR < 0.05 and log 2 fold change magnitude>2.5) in cells co-treated with DCVC and LPS compared to LPS alone. For example, TNF had a log2(fold-change) = -3.5 with the addition of DCVC. Pathways downregulated (adjusted p-value<0.05) in DCVC+LPS treatments versus LPS-only treatments included: "acute inflammatory response", "production of molecular mediator of immune response" and "phagocytosis". LPS increased IL-1ß, IL-6, and TNF-a levels in culture media (p < 0.001), but this was inhibited by co-treatment with DCVC (p < 0.001 for LPS vs. LPS + DCVC treatments). Our results demonstrate that DCVC suppresses inflammatory responses in macrophages. [Description provided by NIOSH]
• Subjects:
  Blood Immune System Macrophages Occupational Health Safety Toxicology
• Keywords:
  Author Keywords: Macrophages Cytokine Cytokines Immune Reaction Inflammation In Vitro Studies Toxicant-pathogen Interactions Trichloroethylene
• ISSN:
  0887-2333
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; Minnesota ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  84
• NIOSHTIC Number:
  nn:20070618
• Citation:
  Toxicol In Vitro 2022 Oct; 84:105429
• Contact Point Address:
  Sean M. Harris, Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029, USA
• Email:
  harrsemi@umich.edu
• CAS Registry Number:
  Trichloroethylene (CAS RN 79-01-6)
• Federal Fiscal Year:
  2023
• Performing Organization:
  University of Michigan, Ann Arbor
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Toxicology in Vitro
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3315f7d03726f397f6fe00f35a29c98c12eaa46a033d8de6280b8276222bf720ebf254a79e16fa060fc60b6e45e774396823d2c3c6cbc49996b1b43e884549d0
• Download URL:
  https://stacks.cdc.gov/view/cdc/208797/cdc_208797_DS1.pdf
• File Type:
  [PDF - 1.67 MB ]