Developmental Programming: Adipose Depot-Specific Regulation of Non-Coding RNAs and Their Relation to Coding RNA Expression in Prenatal Testosterone and Prenatal Bisphenol-a -Treated Female Sheep

Details

• Personal Author:
  Bakulski K ; Dou J ; Elangovan VR ; Padmanabhan V ; Puttabyatappa M ; Thangaraj SV
• Description:
  Inappropriate developmental exposure to steroids is linked to metabolic disorders. Prenatal testosterone excess or bisphenol A (BPA, an environmental estrogen mimic) leads to insulin resistance and adipocyte disruptions in female lambs. Adipocytes are key regulators of insulin sensitivity. Metabolic tissue-specific differences in insulin sensitivity coupled with adipose depot-specific changes in key mRNAs, were previously observed with prenatal steroid exposure. We hypothesized that depot-specific changes in the non-coding RNA (ncRNA) - regulators of gene expression would account for the direction of changes seen in mRNAs. Non-coding RNA (lncRNA, miRNA, snoRNA, snRNA) from various adipose depots of prenatal testosterone and BPA-treated animals were sequenced. Adipose depot-specific changes in the ncRNA that are consistent with the depot-specific mRNA expression in terms of directionality of changes and functional implications in insulin resistance, adipocyte differentiation and cardiac hypertrophy were found. Importantly, the adipose depot-specific ncRNA changes were model-specific and mutually exclusive, suggestive of different regulatory entry points in this regulation. [Description provided by NIOSH]
• Subjects:
  Endocrine System Epidemiology Occupational Health Pregnancy Safety Steroids
• Keywords:
  Adipose Tissue Author Keywords: Fetal Programming Bisphenol A Endocrine Disruptors Insulin Resistance Prenatal Exposure RNA Sequencing
• ISSN:
  0303-7207
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Michigan ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  564
• NIOSHTIC Number:
  nn:20070615
• Citation:
  Mol Cell Endocrinol 2023 Mar; 564:111868
• Contact Point Address:
  Kelly Bakulski, Department of Epidemiology, M5511 SPH II, Ann Arbor, MI, 48109, USA
• Email:
  bakulski@umich.edu
• CAS Registry Number:
  2,2-Bis(4-hydroxyphenyl)propane (CAS RN 80-05-7)
• Federal Fiscal Year:
  2023
• Performing Organization:
  University of Michigan, Ann Arbor
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Molecular and Cellular Endocrinology
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eeca925490c6f703f1aa0e8330aea916dc6fa03c4cf80d3942a11679a88e0761a33001876edec8923ffd5eae7eb8e232ac0b2b23f517b0babee340739e088148
• Download URL:
  https://stacks.cdc.gov/view/cdc/208794/cdc_208794_DS1.pdf
• File Type:
  [PDF - 5.37 MB ]