Proteomic Evaluation of Spore and Hyphae Proteomes of Alternaria alternata

Details

• Personal Author:
  Beezhold, Donald H. ; Fernandes C ; Goncalves T ; Green B ; Hadiwinarta C ; Lemons, Angela R. ; Saha A ; Sharma K ; Strader M ; Beezhold, Donald H. ; Fernandes C ; Goncalves T ; Green B ; Hadiwinarta C ; Lemons, Angela R. ; Saha A ; Sharma K ; Strader M
• Description:
  Rationale: Alternaria alternata is associated with allergic diseases, which can be managed with allergen extract-based diagnostics and immunotherapy. The contributions of asexual spores and vegetative hyphae to patient allergen reactivity are uncertain, and commercial extracts are manufactured by unselective extraction without separating these components. Immunotherapy and diagnosis with existing products, while safe and effective, could be improved by better characterization. Methods: A. alternata spores, germinating spores and hyphae were grown under different conditions and separated by filtration, then extracted and lyophilized for transit. Reconstituted extracts (n=6 for each sample type) were resolved by SDS-PAGE and analyzed by a data independent acquisition strategy using an Orbitrap Fusion Lumos Tribrid Mass Spectrometer. Proteomic differences in allergen profiles and specific ontological groups in each sample type were evaluated using Protalizer software (Vulcan Analytical). Results: We identified 3638 proteins in spores and hyphae, with 157 proteins upregulated in spores and 55 in hyphae. Comparing spores and germinating spores, 23 proteins were upregulated in spores and 43 in germinating spores. These observations were reproducible in all replicates. Sialidases predominated in non-germinating spores. IUIS-identified allergens are more abundant in the non-germinating spore proteome, which consists largely of proteins involved in cell wall synthesis, cellular stress responses and maintaining redox balance and homeostasis. Conclusions: Spores, germinating spores and hyphae have differentially regulated biological pathways. Known A. alternata allergens appear mostly in non-germinating spores. Sialidases might be mechanistically involved in allergen delivery through mucosal surfaces. Information from this study will be important toward understanding allergen reactivity and improving extract potency and specificity. [Description provided by NIOSH]
• Subjects:
  Fungi Hypersensitivity Immune System Occupational Health Safety
• Keywords:
  Allergens Allergic Reactions Allergies Immunology Plants Proteins
• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  151
• Issue:
  2
• NIOSHTIC Number:
  nn:20070238
• Citation:
  J Allergy Clin Immunol 2023 Feb; 151(2)(Suppl):AB197
• Federal Fiscal Year:
  2023
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:54944fb1339e4d4f3d0cf6f3254c6ddfcbeee1485815a06bf3f536bb2222b97ffcea18729b63bc8a9ad57c226b9282dfc652dce6071da29f373488b03a89a8df
• Download URL:
  https://stacks.cdc.gov/view/cdc/208487/cdc_208487_DS1.pdf
• File Type:
  [PDF - 101.91 KB ]