Prevalence of E.coli O157:H7, Salmonella, and Cryptosporidium Among Arizona Dairy Workers Using Post-Work Swabbing

Details

• Personal Author:
  Benally KA ; Cabrera D ; Diaz D ; Lopez G ; Lopez-Galvez NI ; Wagoner R ; Benally KA ; Cabrera D ; Diaz D ; Lopez G ; Lopez-Galvez NI ; Wagoner R
• Description:
  The dairy industry in Arizona, like many other agricultural industries in the United States, is dependent on the labor that migrant farm workers provide. Infections caused by zoonotic pathogens are commonly underreported or misdiagnosed, and possibly more so in migratory workers that face cultural, structural, legal, financial, and geographic barriers to health services. The objectives of this project were to: assess the demographics of Arizona dairy workers, determine the exposure potential of Arizona dairy workers to zoonotic organisms, and inform best management practices. A questionnaire including demographics, work tasks, and household characteristics was administered. Swab samples were collected from the shoulders, knees, and foreheads of employees at two dairy operations at the end of the work shift. The swabs were cultured for E.coli O157:H7 and Salmonella. Molecular DNA isolated from Salmonella and Cryptosporidium was quantified using droplet-digital Polymerase Chain Reaction (ddPCR). Twenty dairy workers were recruited, and 60 samples were collected. The majority of workers were male, preferred to speak Spanish, and identified as Latino/Hispanic (68.8%, 93.8%, and 93.8%, respectively). E. coli O157:H7 was detected in 13% of cultured knee and forehead samples. Salmonella spp. gene copies were detected on 60.0% of samples collected from forehead skin samples; 40.0% of shoulder clothing samples; and 15% of knee clothing samples, as measured via ddPCR. The positive cultural and molecular samples indicate the need for improved post-workday sanitation practices at farms. This study provides surveillance of a largely invisible population, including insights that can be used to create site-specific health and safety protocols for the dairy industry, inform risk assessment models, and foster preventive practices in the dairy industry. [Description provided by NIOSH]
• Subjects:
  Agriculture Emigration And Immigration Farmers Occupational Health Safety
• Keywords:
  Agricultural Industry Animal Husbandry Workers Author Keywords: Agricultural Workers Bacterial Disease Dairy Workers DdPCR Exposure Farm Workers Microbial Migrant Workers Surveillance Zoonoses Zoonotic

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• ISSN:
  1074-7583
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  Arizona ; California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  17-34
• Volume:
  30
• Issue:
  1
• NIOSHTIC Number:
  nn:20069876
• Citation:
  J Agric Saf Health 2024 Jan; 30(1):17-34
• Contact Point Address:
  Duarte Diaz, School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ, United States
• Email:
  duartediaz@arizona.edu
• Federal Fiscal Year:
  2024
• NORA Priority Area:
  Agriculture, Forestry and Fishing ; Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Agricultural Safety and Health
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:523383efb9be20e58501591d30331e85d3c7a509ca41df94d7817ff244c650b748ce13755ae611de6c5b10b34c5ce10431c124179977c49b7ab774703c0db359
• Download URL:
  https://stacks.cdc.gov/view/cdc/208216/cdc_208216_DS1.pdf
• File Type:
  [PDF - 408.34 KB ]