Wastewater Sequencing Reveals Community and Variant Dynamics of the Collective Human Virome

Details

• Personal Author:
  Avadhanula V ; Ayvaz T ; Balliew J ; Bauer CX ; Boerwinkle E ; Clark JR ; Cormier J ; Deegan J ; Feliz K ; Gitter A ; Hansen B ; Henke D ; Hoffman KL ; Javornik Cregeen S ; Li R ; Maresso AW ; Mena KD ; Moreno H ; Payne K ; Petrosino JF ; Piedra PA ; Rios J ; Ross MC ; Terwilliger A ; Tisza M ; Troisi C ; Wang L ; Wu F ; Zhang K ; Zhang P
• Description:
  Wastewater is a discarded human by-product, but its analysis may help us understand the health of populations. Epidemiologists first analyzed wastewater to track outbreaks of poliovirus decades ago, but so-called wastewater-based epidemiology was reinvigorated to monitor SARS-CoV-2 levels while bypassing the difficulties and pit falls of individual testing. Current approaches overlook the activity of most human viruses and preclude a deeper understanding of human virome community dynamics. Here, we conduct a comprehensive sequencing-based analysis of 363 longitudinal wastewater samples from ten distinct sites in two major cities. Critical to detection is the use of a viral probe capture set targeting thousands of viral species or variants. Over 450 distinct pathogenic viruses from 28 viral families are observed, most of which have never been detected in such samples. Sequencing reads of established pathogens and emerging viruses correlate to clinical data sets of SARS-CoV-2, influenza virus, and monkeypox viruses, outlining the public health utility of this approach. Viral communities are tightly organized by space and time. Finally, the most abundant human viruses yield sequence variant information consistent with regional spread and evolution. We reveal the viral landscape of human wastewater and its potential to improve our understanding of outbreaks, transmission, and its effects on overall population health. [Description provided by NIOSH]
• Subjects:
  Communicable Diseases Epidemiology Influenza, Human Occupational Health Safety Viral Diseases
• Keywords:
  Disease Transmission Emerging Infectious Diseases Infectious Diseases Influenza Longitudinal Study Monkeypox Mpox Pathogens SARS-CoV-2 Viral Diseases Water Sampling

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• ISSN:
  2041-1723
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• NIOSHTIC Number:
  nn:20069750
• Citation:
  Nat Commun 2023 Oct; 14:6878
• Contact Point Address:
  Anthony W. Maresso, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, 77030, USA
• Email:
  maresso@bcm.edu
• Federal Fiscal Year:
  2024
• Performing Organization:
  University of Texas Health Science Center, Houston
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Nature Communications
• End Date:
  20250630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9857dadfecc7684854ad8fa27014745e69001e167d6cbb67b1ab944f533ceb77e07d3cbb75b86b40d663119b1e96bf596bda35f8e3e38f41c630dad3521208f3
• Download URL:
  https://stacks.cdc.gov/view/cdc/208112/cdc_208112_DS1.pdf
• File Type:
  [PDF - 72.42 MB ]