Urine Proteome Profile of Firefighters with Exposure to Emergency Fire-Induced Smoke: A Pilot Study to Identify Potential Carcinogenic Effects

Details

• Personal Author:
  Agnew RJ ; Ahsan N ; Hwang J ; Najar FZ ; Peng Z ; Xu C ; Xu X ; Yang Z
• Description:
  Firefighters are frequently exposed to a variety of chemicals formed from smoke, which pose a risk for numerous diseases, including cancer. Comparative urine proteome profiling could significantly improve our understanding of the early detection of potential cancer biomarkers. In this study, for the first time, we conducted a comparative protein profile analysis of 20 urine samples collected from ten real-life firefighters prior to and following emergency fire-induced smoke. Using a label-free quantitative proteomics platform, we identified and quantified 1325 unique protein groups, of which 45 proteins showed differential expressions in abundance in response to fire-smoke exposure (post) compared to the control (pre). Pathway analysis showed proteins associated with epithelium development (e.g., RHCG, HEG1, ADAMTSL2) and Alzheimer's disease (SORL1) were significantly increased in response to smoke exposure samples. A protein-protein-network study showed a possible link between these differentially abundant proteins and the known cancer gene (TP53). Moreover, a cross-comparison analysis revealed that seven proteins-ALDH1A1, APCS, POMC, COL2A1, RDX, DDAH2, and SDC4 overlapped with the previously published urine cancer proteome datasets, suggesting a potential cancer risk. Our findings demonstrated that the discovery proteomic platform is a promising analytical technique for identifying potential non-invasive biomarkers associated with fire-smoke exposure in firefighters that may be related to cancer. [Description provided by NIOSH]
• Subjects:
  Biomarkers Carcinogens Firefighters Neoplasms Occupational Health Safety Urine
• Keywords:
  Author Keywords: Firefighters Cancer Fire Fighters Fire Smoke Proteins Proteomic Smoke Inhalation
• ISSN:
  0048-9697
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Oklahoma ; OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  927
• NIOSHTIC Number:
  nn:20069601
• Citation:
  Sci Total Environ 2024 Jun; 927:172273
• Contact Point Address:
  N. Ahsan, Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, USA
• Email:
  nahsan@ou.edu
• Federal Fiscal Year:
  2024
• Performing Organization:
  University of Oklahoma Health Sciences Center, Oklahoma City
• Peer Reviewed:
  True
• Start Date:
  20200901
• Source Full Name:
  Science of the Total Environment
• End Date:
  20210831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:653e3632e7f384ef88451b5982873d09161dbb62b11e67361ed86e0b94c982f8f6ca101ed924cfea02638d4053200d7190fbe641c7486422dafd1e2d35b733ba
• Download URL:
  https://stacks.cdc.gov/view/cdc/208001/cdc_208001_DS1.pdf
• File Type:
  [PDF - 5.38 MB ]