Impact of Zinc Supplementation on Phenotypic Antimicrobial Resistance of Fecal Commensal Bacteria from Pre-Weaned Dairy Calves

Details

• Personal Author:
  Aly SS ; Atwill ER ; Feldmann HR ; Lee KY ; Li X ; Weimer BC ; Williams DR
• Description:
  The objective of this study was to evaluate the impact of dietary zinc supplementation in pre-weaned dairy calves on the phenotypic antimicrobial resistance (AMR) of fecal commensal bacteria. A repository of fecal specimens from a random sample of calves block-randomized into placebo (n= 39) and zinc sulfate (n= 28) groups collected over a zinc supplementation clinical trial at the onset of calf diarrhea, calf diarrheal cure, and the last day of 14 cumulative days of zinc or placebo treatment were analyzed. Antimicrobial susceptibility testing was conducted for Enterococcus spp. (n= 167) and E. coli (n= 44), with one representative isolate of each commensal bacteria tested per sample. Parametric survival interval regression models were constructed to evaluate the association between zinc treatment and phenotypic AMR, with exponentiated accelerated failure time (AFT) coefficients adapted for MIC instead of time representing the degree of change in AMR (MIC Ratio, MR). Findings from our study indicated that zinc supplementation did not significantly alter the MIC in Enterococcus spp. for 13 drugs: gentamicin, vancomycin, ciprofloxacin, erythromycin, penicillin, nitrofurantoin, linezolid, quinupristin/dalfopristin, tylosin tartrate, streptomycin, daptomycin, chloramphenicol, and tigecycline (MR= 0.96-2.94, p > 0.05). In E. coli, zinc supplementation was not associated with resistance to azithromycin (MR= 0.80, p > 0.05) and ceftriaxone (MR= 0.95, p > 0.05). However, a significant reduction in E. coli MIC values was observed for ciprofloxacin (MR= 0.17, 95% CI 0.03-0.97) and nalidixic acid (MR= 0.28, 95% CI 0.15-0.53) for zinc-treated compared to placebo-treated calves. Alongside predictions of MIC values generated from these 17 AFT models, fundings from this study corroborate the influence of age and antimicrobial exposure on phenotypic AMR. [Description provided by NIOSH]
• Subjects:
  Agriculture Cattle Livestock Occupational Health Safety
• Keywords:
  Antimicrobial Resistance Antimicrobials Bacteria Cattle Industry Zinc
• ISSN:
  2045-2322
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• NIOSHTIC Number:
  nn:20069281
• Citation:
  Sci Rep 2024 Feb; 14:4448
• Contact Point Address:
  Sharif S. Aly, Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, Davis, CA, USA
• Email:
  saly@ucdavis.edu
• CAS Registry Number:
  Zinc (CAS RN 7440-66-6)
• Federal Fiscal Year:
  2024
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Scientific Reports
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ecc8f1c6a5d1a16b5942f6a483e25bd8e60f464329913790812a4a68747e6721b3848065488e0bad5bfbc24d01b66cc0b27ffe2ea8ace57f23b1db04f5336588
• Download URL:
  https://stacks.cdc.gov/view/cdc/207785/cdc_207785_DS1.pdf
• File Type:
  [PDF - 4.12 MB ]