Optimization of Aspergillus versicolor Culture and Aerosolization in a Murine Model of Inhalational Fungal Exposure

Details

• Personal Author:
  Anderson S ; Barnes MA ; Beezhold, Donald H. ; Blackwood CB ; Croston TL ; Germolec D ; Goldsmith T ; Green B ; Lemons, Angela R. ; McKinney WG ; Park, Ju-Hyeong ; Rush RE ; Sulyok M ; Weaver KL
• Description:
  Aspergillus versicolor is ubiquitous in the environment and is particularly abundant in damp indoor spaces. Exposure to Aspergillus species, as well as other environmental fungi, has been linked to respiratory health outcomes, including asthma, allergy, and even local or disseminated infection. However, the pulmonary immunological mechanisms associated with repeated exposure to A. versicolor have remained relatively uncharacterized. Here, A. versicolor was cultured and desiccated on rice then placed in an acoustical generator system to achieve aerosolization. Mice were challenged with titrated doses of aerosolized conidia to examine deposition, lymphoproliferative properties, and immunotoxicological response to repeated inhalation exposures. The necessary dose to induce lymphoproliferation was identified, but not infection-like pathology. Further, it was determined that the dose was able to initiate localized immune responses. The data presented in this study demonstrate an optimized and reproducible method for delivering A. versicolor conidia to rodents via nose-only inhalation. Additionally, the feasibility of a long-term repeated exposure study was established. This experimental protocol can be used in future studies to investigate the physiological effects of repeated pulmonary exposure to fungal conidia utilizing a practical and relevant mode of delivery. In total, these data constitute an important foundation for subsequent research in the field. [Description provided by NIOSH]
• Subjects:
  Asthma Asthma, Occupational Fungi Hypersensitivity Occupational Health Respiratory System Safety
• Keywords:
  Aerosolization Allergen Allergies Aspergillus Infection Aspergillus Versicolor Author Keywords: Aspergillus Bioaerosols Indoor Environments Inhalation Murine Nose-only Exposure Repeated Indoor Fungal Exposure Respiratory Health

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• ISSN:
  2309-608X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  9
• Issue:
  11
• NIOSHTIC Number:
  nn:20068820
• Citation:
  J Fungi 2023 Nov; 9(11):1090
• Contact Point Address:
  Tara L. Croston, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505
• Email:
  xzu9@cdc.gov
• Federal Fiscal Year:
  2024
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Fungi
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2612a2089fff61d9bb4a41e3b2564db8a6d7ace656eddb4a540fc3faa2af96d90ca9d7152e4b049249656e08ad2426a2944ae1fb398688e7cc7c2a799fb30137
• Download URL:
  https://stacks.cdc.gov/view/cdc/207394/cdc_207394_DS1.pdf
• File Type:
  [PDF - 2.67 MB ]