Respiratory Exposure to Agricultural Dust Extract Promotes Increased Intestinal Tnfa Expression, Gut Barrier Dysfunction, and Endotoxemia in Mice

Details

• Personal Author:
  Canale V ; Chatterjee P ; Crawford MS ; Lei H ; Manz S ; McCole DF ; Nordgren TM ; Ramirez BM ; Santos AN ; Ulu A
• Description:
  Concentrated animal feeding operations (CAFOs) are responsible for the production of global greenhouse gases and harmful environmental pollutants including hydrogen sulfide, ammonia, and particulate matter. Swine farmers are frequently exposed to organic dust that is proinflammatory in the lung and are thus at greater risk of developing pneumonia, asthma, and other respiratory conditions. In addition to respiratory disease, air pollutants are directly associated with altered gastrointestinal (GI) physiology and the development of GI diseases, thereby highlighting the gut-lung axis in disease progression. Instillation of hog dust extract (HDE) for 3 wk has been reported to promote the development of chronic airway inflammation in mice, however, the impact of HDE exposure on intestinal homeostasis is poorly understood. We report that 3-wk intranasal exposure of HDE is associated with increased intestinal macromolecule permeability and elevated serum endotoxin concentrations in C57BL/6J mice. In vivo studies also indicated mislocalization of the epithelial cell adhesion protein, E-cadherin, in the colon as well as an increase in the proinflammatory cytokine, Tnfa, in the proximal colon. Moreover, mRNA expression of the Paneth cell-associated marker, Lyz1, was increased the proximal colon, whereas the expression of the goblet cell marker, Muc2, was unchanged in the epithelial cells of the ileum, cecum, and distal colon. These results demonstrate that airway exposure to CAFOs dusts promote airway inflammation and modify the gastrointestinal tract to increase intestinal permeability, induce systemic endotoxemia, and promote intestinal inflammation. Therefore, this study identifies complex physiological consequences of chronic exposure to organic dusts derived from CAFOs on the gut-lung axis. [Description provided by NIOSH]
• Subjects:
  Agriculture Air Pollutants, Occupational Digestive System Digestive System Diseases Dust Endotoxins Immune System Occupational Health Safety
• Keywords:
  Agricultural Industry Air Pollutants Animal Husbandry Workers Author Keywords: Agricultural Pollution Cytokines Gastrointestinal System Disorders Gut-lung Axis Immune Reaction Intestinal Barrier Function LPS Organic Dusts

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• ISSN:
  0193-1857
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Colorado ; OSHA Region 8 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  326
• Issue:
  1
• NIOSHTIC Number:
  nn:20068683
• Citation:
  Am J Physiol Gastrointest Liver Physiol 2024 Jan; 326(1):G3-G15
• Contact Point Address:
  Declan F. McCole, School of Medicine, Division of Biomedical Sciences, University of California, 10 Riverside, Riverside, CA
• Email:
  declan.mccole@ucr.edu
• Federal Fiscal Year:
  2024
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  American Journal of Physiology: Gastrointestinal and Liver Physiology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9cabfd31d5ec92a980cf6e0973f12bd4e52366fa0a4852f539fb14815173e0d4ced954e1efac52a4774680d59c6892de0e3763ea1c6fd4d1525ad2bd9ca7729b
• Download URL:
  https://stacks.cdc.gov/view/cdc/207273/cdc_207273_DS1.pdf
• File Type:
  [PDF - 4.89 MB ]