Reactivity of guinea-pig isolated trachea to methacholine, histamine and isoproterenol applied serosally versus mucosally

Details

• Personal Author:
  Fedan, Jeffery S. ; Frazer DG ; Nutt ME
• Description:
  The reactivity of the trachealis smooth muscle of the isolated, perfused male English-guinea-pig trachea to methacholine (62511), histamine (51456) and isoproterenol (7683592) was compared after the drugs were applied to the intraluminal (IL) or extraluminal (EL) surfaces. All agents were more potent when added to the EL component but the IL/EL median effective concentration ratios were different being 100 for methacholine, 41 for histamine, and 25 for isoproterenol. Methacholine or histamine added to the IL compartment, after the preparations were precontracted with the same concentration of the agonist of 30 millimolar potassium-chloride (KCL) added EL, did not result in relaxation. Similarly IL isoproterenol did not evoke contraction. IL KCl evoked relaxation. The authors conclude that the epithelium reduces access of bronchoactive agents to the muscle while an immediate relaxant effect of epithelium derived relaxing factor released by agonists could not be demonstrated. [Description provided by NIOSH]
• Subjects:
  Biopharmaceutics Laboratories Lung Musculoskeletal System Occupational Health Respiratory System Safety
• Keywords:
  Airway-resistance In-vitro-study Laboratory-animals Lung-irritants Muscle-function Muscle-physiology Muscle-relaxants NIOSH-Author Pharmaceuticals Pulmonary-system
• ISSN:
  0014-2999
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  337-345
• Volume:
  190
• Issue:
  3
• NIOSHTIC Number:
  nn:00204900
• Citation:
  Eur J Pharmacol 1990 Nov; 190(3):337-345
• CAS Registry Number:
  Histamine (CAS RN 51-45-6) ; Isoproterenol (CAS RN 7683-59-2) ; Methacholine chloride (CAS RN 62-51-1)
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  True
• Source Full Name:
  European Journal of Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7bf218cf51fb0682d3b41300348094b1ce66db4fc1492f588b87c62ffd8d031bbdda8bac1c6dd33003db0154c3bc08628d3dd5d3dba9f7cbee9266bb4e4b50fe
• Download URL:
  https://stacks.cdc.gov/view/cdc/205459/cdc_205459_DS1.pdf
• File Type:
  [PDF - 1.00 MB ]