Primary contribution of the injector to carryover of a trace analyte in high-performance liquid chromatography

Details

• Personal Author:
  Giese RW ; Saha M
• Description:
  A study was undertaken to determine the extent to which the injector contributes to the problem of analyte carryover in high performance liquid chromatography (HPLC). Two experiments were conducted to measure a low nanogram amount of N1,N3-bis(pentafluorobenzyl)-N7-(2- (pentafluorobenzyloxy)ethyl)xanthine using HPLC. Despite immediate washing of the injector and HPLC column, analyte carryover was seen after injection of the pure mobile phase. Possible mechanisms for analyte retention in the injector were suspected to be active sites and/or solvent dead volumes. Comparable carryover was seen in trials where gradients were conducted immediately following installation of the clean injector, indicating that the isocratic washing procedure was as effective as gradient conduction. Total carryover in all trials remained below 1% of the originally injected analyte. From this study the authors conclude that the use of two injectors each dedicated to different analyte levels can alleviate the problem of injector induced analyte carryover in HPLC. [Description provided by NIOSH]
• Subjects:
  Chromatography Chromatography, Gas Occupational Health Safety
• Keywords:
  Analytical-methods Analytical-processes Chromatographic-analysis Gas-liquid-chromatography Grants-other NIOSH-Grant NIOSH-Publication Sample-preparation Trace-analysis
• ISSN:
  0021-9673
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maine ; Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  161-163
• Volume:
  631
• Issue:
  1
• NIOSHTIC Number:
  nn:00232471
• Citation:
  J Chromatogr A 1993 Feb; 631(1/2):161-163
• Contact Point Address:
  Medicinal Chemistry Northeastern University 360 Huntington Avenue Boston, MA 02115
• CAS Registry Number:
  1H-Purine-2,6-dione, 3,7-dihydro-7-[2-[(pentafluorophenyl)methoxy]ethyl]-1,3-bis[(pentafluorophenyl)methyl]- (9CI) (CAS RN 126565-13-7)
• Federal Fiscal Year:
  1993
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  Northeastern University, Boston, Massachusetts
• Peer Reviewed:
  True
• Start Date:
  19910101
• Source Full Name:
  Journal of Chromatography A
• End Date:
  19961130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1c925357d60f1b5de5568ae4c00ce9de4d2ba48e1d3cbdc0559d53f3932fb59af78430519904e2bdc90e238470137ab24ec6f439b570b064c9cc5b1a98d1c927
• Download URL:
  https://stacks.cdc.gov/view/cdc/205401/cdc_205401_DS1.pdf
• File Type:
  [PDF - 235.89 KB ]