Pharmacologic characterization of wool dust extract in isolated guinea pig trachea

Details

• Personal Author:
  Buck MG ; Goswami S ; Maayani S ; Marom Z ; Rienzi N ; Schachter EN ; Zuskin E
• Description:
  The airway effects of wool dust were examined in guinea-pig tracheal rings and compared with previous results obtained for cotton bract extract (CBE). Wool dust was collected from carding machines at a mill in Croatia. Wool dust extract (WDE) was prepared by incubating wool with sterile distilled water in a weight to volume ratio of 1 gram wool dust to 10 milliliters sterile water for 24 hours. Tracheal tissue came from male albino-Hartley-guinea-pigs. Four segments each 4 to 6 millimeters wide, were cut from a single trachea and suspended between two L-shaped stainless steel hooks in an organ chamber. Dose response curves to WDE were measured in 30 guinea-pig tracheal rings obtained from different animals and expressed as a percentage of maximal carbachol contraction. WDE produced bronchoconstriction defined by a dose response curve similar to CBE. The maximal muscle tension for WDE was 57.9%, compared with 42.3% for CBE. Pyrilamine partially blocked the constricting effect of WDE at the highest WDE doses. Verapamil and trimethoxybenzoic-acid completely blocked the response to WDE. In contrast, CBE induced bronchoconstriction was significantly inhibited by a number of chemical agents, including atropine, indomethacin, and pyrilamine. Endotoxin was not present in the WDE. The authors conclude that WDE may have clinical effects related to non immunoglobulin-E mechanisms, which could have therapeutic implications for respiratory symptoms in wool textile workers. [Description provided by NIOSH]
• Subjects:
  Laboratories Occupational Health Respiratory Hypersensitivity Respiratory Tract Diseases Safety Textile Industry Wool
• Keywords:
  In-vitro-study Laboratory-animals NIOSH-Grant NIOSH-Publication Pulmonary-system-disorders Respiratory-hypersensitivity Textile-mills Wools
• ISSN:
  0013-9351
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  90-95
• Volume:
  69
• Issue:
  2
• NIOSHTIC Number:
  nn:00232238
• Citation:
  Environ Res 1995 May; 69(2):90-95
• Contact Point Address:
  Work Environment Univ of Lowell Research Fdn 450 Aiken St Lowell, MA 01854
• Federal Fiscal Year:
  1995
• NORA Priority Area:
  Pulmonary System Disorders
• Performing Organization:
  University of Massachusetts Lowell, Lowell, Massachusetts
• Peer Reviewed:
  True
• Start Date:
  19920401
• Source Full Name:
  Environmental Research
• End Date:
  19960831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7eb05ad86e75b48e37e3c38178c0242648bed9fad510932d2c58757d32b1def6fa2e03dcf03f554c57fc58cb762d15f77785d903c0298b50d7b98267f4a2db4a
• Download URL:
  https://stacks.cdc.gov/view/cdc/205361/cdc_205361_DS1.pdf
• File Type:
  [PDF - 476.66 KB ]