Maintenance of glutathione levels in renal cortex slices of the rat

Details

• Personal Author:
  Murphy SD ; Richardson RJ ; Wilder AC
• Description:
  The stability of glutathione concentrations in rat kidney cortex slices was examined. Renal cortex slices obtained from male Holtzman-rats were incubated in Krebs-Ringer-bicarbonate (KRB) buffer under 95 percent oxygen and 5 percent carbon-dioxide in the presence or absence of 4 percent bovine serum albumin (BSA) at 37 degrees-C for 30 to 120 minutes. The effects on the glutathione content of the slices were determined. Glutathione concentrations decreased during the incubation period. BSA had no effect on the rate of glutathione disappearance. Rat kidney slices were incubated in KRB buffer containing 0 or 3 millimolar disodium-maleate (371471) for 10 to 120 minutes and the effects on glutathione content were determined. Disodium-maleate had no effect on the disappearance of glutathione between 10 and 60 minutes of incubation; however, for incubation periods of 90 to 120 minutes the rate of decrease was accelerated. Rat kidney slices were incubated with dithiothreitol (27565419), glycine (56406), glutamic-acid (56860), methionine (63683), cysteine (52904), or glutamine (56859) alone or in combination for 60 minutes and the effects on their glutathione content were assessed. Dithiothreitol alone had little or no effect on glutathione. Cysteine alone increased the concentration of glutathione, an effect which was enhanced by dithiothreitol. Methionine alone decreased glutathione content. The other amino acids by themselves had little effect, but when given together they increased the glutathione content. Similar experiments were conducted in which the incubation atmosphere was nitrogen instead of oxygen, the incubation temperature was lowered to 25 and 0 degrees- C, and the oxidative phosphorylation uncoupler dinitrophenol (573568) was present. Conducting the incubation under nitrogen or in the presence of dinitrophenol decreased the glutathione content, whereas lowering the temperature increased glutathione content. The authors conclude that the concentration of glutathione in rat kidney slices depends on overall metabolism and the availability of the component amino acids of glutathione. Enzymatic degradation of glutathione can occur anaerobically, whereas oxidative metabolism is required for glutathione synthesis. [Description provided by NIOSH]
• Subjects:
  Amino Acids Biological Products Laboratories Occupational Health Safety Sulfur Compounds
• Keywords:
  Amino-acids Biological-material Biotransformation Chemical-kinetics Grants-other In-vitro-studies Laboratory-techniques NIOSH-Grant NIOSH-Publication Organo-sulfur-compounds Physiological-chemistry

  More +
• ISSN:
  0037-9727
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  360-364
• Volume:
  154
• Issue:
  3
• NIOSHTIC Number:
  nn:00186809
• Citation:
  Proc Soc Exp Biol Med 1977 Jan; 154(3):360-364
• Contact Point Address:
  Physiology Harvard University 665 Huntington Ave Boston, Mass 02115
• CAS Registry Number:
  2,6-Dinitrophenol (CAS RN 573-56-8) ; Disodium maleate (CAS RN 371-47-1) ; Dithiothreitol (CAS RN Dithiothreitol (CAS RN 3483-12-3)) ; Glycine (CAS RN 56-40-6) ; L-(+)-Cysteine (CAS RN 52-90-4) ; L-Glutamic acid (CAS RN 56-86-0) ; L-Glutamine (CAS RN 56-85-9) ; l-Methionine (CAS RN 63-68-3)
• Federal Fiscal Year:
  1977
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  Harvard University, Boston, Massachusetts
• Peer Reviewed:
  True
• Start Date:
  19710101
• Source Full Name:
  Proceedings of the Society for Experimental Biology and Medicine
• End Date:
  19810430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1e235ff11df44732a7150f93bd2e1e56e542149ec1d97cd7e38134a7d24d5ddad7da537b964c3015e825f3b010e468459286ce89dc053c24203ec588b27e582a
• Download URL:
  https://stacks.cdc.gov/view/cdc/205058/cdc_205058_DS1.pdf
• File Type:
  [PDF - 523.68 KB ]