Macrophage regulation of myelopoiesis is altered by exposure to the benzene metabolite hydroquinone

Details

• Personal Author:
  Reasor MJ ; Thomas DJ ; Wierda D
• Description:
  Efforts were made to determine whether decreased support of myelopoiesis by bone marrow stroma after in-vitro hydroquinone (123319) exposure was due to selective toxicity to either bone marrow macrophages or fibroblastoid stromal cells. Mouse bone marrow was used to obtain independent populations of fibroblastoid stromal cells and macrophages which were then exposed in culture to hydroquinone. These cells were reconstituted in culture and assayed for their ability to support granulocyte/macrophage colony formation. Bone marrow derived fibroblastoid stromal cell line identified as LTF and primary fibroblastoid stromal cells derived from bone marrow mixed cultures over a 14 day period were both used in the study. Reconstituted cultures containing hydroquinone treated macrophages supported fewer colonies than did corresponding cultures containing untreated macrophages. Reconstituted cultures containing hydroquinone treated fibroblastoid stromal cells were not affected. Hydroquinone reduced detectable interleukin-1 activity in purified macrophage cultures stimulated with lipopolysaccharide. The findings suggest that bone marrow macrophages, rather than bone marrow fibroblastoid stromal cells, are targets for hydroquinone myelotoxicity. Hydroquinone may selectively interfer with macrophage function, perhaps, by altering macrophage interleukin-1 secretion. The findings suggested that replacement of certain growth factors may be feasible in therapy of patients who present with myelotoxic symptoms caused by exposure to benzene (71432) or other chemical agents. [Description provided by NIOSH]
• Subjects:
  Benzene Carcinogens Cells, Cultured Energy Metabolism Occupational Health Poisoning Safety
• Keywords:
  Benzene-poisoning Carcinogenesis Cell-cultures Grants-other Hematopoietic-system-disorders Metabolites Myeloid-tissue NIOSH-Grant NIOSH-Publication
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  440-453
• Volume:
  97
• Issue:
  3
• NIOSHTIC Number:
  nn:00188094
• Citation:
  Toxicol Appl Pharmacol 1989 Mar; 97(3):440-453
• Contact Point Address:
  Pharmacology and Toxicology West VA Univ Medical Center Dept of Pharmacology & Toxicol Morgantown, W VA 26506
• CAS Registry Number:
  Benzene (CAS RN 71-43-2) ; Hydroquinone (CAS RN 123-31-9)
• Federal Fiscal Year:
  1989
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  True
• Start Date:
  19830401
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19900831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d755e39e213d58aa72207a3600969d5c70725707332fdacbae0f80a3407c9834a94222ee783a367db9af47dc456474e2e3dbe35348072222c35d9f4a31a69922
• Download URL:
  https://stacks.cdc.gov/view/cdc/205057/cdc_205057_DS1.pdf
• File Type:
  [PDF - 894.20 KB ]