Isolation of metal-binding agents from coal dust and their effects on mitochondrial function

Details

• Personal Author:
  Elia VJ ; Murthy L ; Petering HG
• Description:
  A study was undertaken to develop a method for extracting coal dust, to establish whether the extracts contained metal binding agents, and to gather evidence that these agents may interfere with trace metal metabolism or act as inhibitors of metalloenzymes requiring biological systems. Liver mitochondrial samples were prepared using Sprague-Dawley-rats. Two types of bituminous coal were used. The first, from Pennsylvania, was a harder variety, contained more heavy metals and caused a higher incidence of coal miner's pneumoconiosis than the second sample which was from Utah. The organic extract from the Utah sample contained material equivalent to 58 to 70 milligrams/gram (mg/g) of coal. The Pennsylvania sample extract contained material equivalent to 12 to 15mg/g of coal. The whole organic extract from each coal was chromatographed on carboxymethyl- cellulose in Cu(II) form (CMC-CuII). For Utah and Pennsylvania samples, the copper complexing fractions were 30 and 22 percent, respectively, of the total extract. Copper binding capacity was 400 micrograms copper/gram of Utah coal and 40 micrograms/gram Pennsylvania coal. The oxidative phosphorylation of isolated rat liver mitochondria was uncoupled by copper binding ligands from both coals. The Pennsylvania sample had a higher inhibitory activity per gram of extracted material. The authors suggest that the extraction and absorption of metal binding agents from coal may adversely alter vital cellular energetics. [Description provided by NIOSH]
• Subjects:
  Coal Coal Mining Dust Energy Metabolism Laboratories Lung Lung Diseases Mining Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Cell-function Coal-dust Coal-mining Grants-other In-vitro-studies Laboratory-animals Lung-cells Lung-disorders Metabolic-study Mining-industry NIOSH-Grant NIOSH-Publication Pulmonary-system-disorders

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• ISSN:
  0013-9300
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 8 ; Pennsylvania ; Utah
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  237-248
• Volume:
  5
• Issue:
  4
• NIOSHTIC Number:
  nn:00190455
• Citation:
  Environ Lett 1973 Oct-Dec; 5(4):237-248
• Contact Point Address:
  Environmental Health University of Cincinnati Eden & Bethesda Avenues Cincinnati, Ohio 45219
• Federal Fiscal Year:
  1974
• NORA Priority Area:
  Grants Other
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19710601
• Source Full Name:
  Environmental Letters
• End Date:
  19771231
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fdc773b431c0c0923c3dc7f80e60b68864877969d2c0f8f8dbc31f16b74c2ebbcf2dc8fb52ab5cf06f2e6322d044c962b3b360612f463e92a0a20f120a1a98ed
• Download URL:
  https://stacks.cdc.gov/view/cdc/204989/cdc_204989_DS1.pdf
• File Type:
  [PDF - 358.92 KB ]