Influence of the dose levels of cocarcinogen ferric oxide on the metabolism of benzo(a)pyrene by pulmonary alveolar macrophages in suspension culture

Details

• Personal Author:
  Greife AL ; Warshawsky D
• Description:
  The effects of the phagocytosis of ferric-oxide (1309371) (FeO) by alveolar macrophages on the metabolism of benzo(a)pyrene (50328) (BaP) and on the production of reactive oxygen species were studied in-vitro. Alveolar macrophages were harvested by tracheal lavage from Syrian-Golden-hamsters, cultured with dimethylsulfoxide and cytochrome-c, and exposed to radiolabeled BaP that had previously been adsorbed onto FeO. A decrease in cell viability was seen that was dependent upon the dose of BaP coated particles present in the culture media. A significant increase in the amount of reduced cytochrome-c was seen up to 6 hours after the addition of BaP coated FeO or FeO alone compared with the amount reduced in untreated cells or cells incubated with BaP alone. The amount of reduced cytochrome- c plateaued in all preparations after 6 hours. The highest concentration of phagocytosed BaP was seen in cultures exposed to BaP coated FeO at 3 hours. The phagocytosed BaP was metabolized by the macrophages and the total concentration of metabolites produced appeared to be dependent on the dose of FeO. Significantly more metabolites were found in cells exposed to BaP coated FeO compared with those exposed to BaP alone. [Description provided by NIOSH]
• Subjects:
  Carcinogens Energy Metabolism Lung Occupational Health Pyrenes Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Dose-response In-vitro-study Lung-cells Mammalian-cells Metabolic-study NIOSH-Author NIOSH-Grant NIOSH-Publication Oxidative-processes Pulmonary-system-disorders
• ISSN:
  0098-4108
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  399-417
• Volume:
  38
• Issue:
  4
• NIOSHTIC Number:
  nn:00213986
• Citation:
  J Toxicol Environ Health 1993 Apr; 38(4):399-417
• Contact Point Address:
  Environmental Health University of Cincinnati 3223 Eden Ave - M L #56 Cincinnati, OH 45267-0056
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8) ; Iron oxide (Fe2O3) (CAS RN 1309-37-1)
• Federal Fiscal Year:
  1993
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19860701
• Source Full Name:
  Journal of Toxicology and Environmental Health
• End Date:
  19970331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f2013ca71d1f380aea8091f4046f0a20540c6d0e55416e097409b552ab1d7d353e3a95f6d00980aa8b4fb2915330ed15dff7aa87d5f202298015299e13abcc57
• Download URL:
  https://stacks.cdc.gov/view/cdc/204935/cdc_204935_DS1.pdf
• File Type:
  [PDF - 1.10 MB ]