Evidence that lipid peroxidation in microsomal membranes of epidermis is associated with generation of hydrogen peroxide and singlet oxygen

Details

• Personal Author:
  Bickers DR ; Dixit R ; Mukhtar H
• Description:
  Evidence was presented which indicated that hydrogen-peroxide and singlet oxygen may mediate peroxidation of microsomal lipids of the epidermis. Neonatal Sprague-Dawley-rats were sacrificed on day four after birth, and epidermal microsomes were prepared from the skin. Incubation of epidermal microsomes with NADPH or xanthine-oxidase system resulted in significant generation of lipid peroxides as measured by malondialdehyde formation. Epidermal lipid peroxidation was inhibited by catalase and singlet oxygen quenchers, 2,5- dimethylfuran, histidine and beta-carotene whereas hydroxide ion scavengers, benzoate, mannitol and ethyl-alcohol had no effect. No protective effect was noted by superoxide-dismutase on lipid peroxidation. Vitamin-E and cytochrome-C3+ substantially inhibited the epidermal lipid peroxidation. The authors suggest that polyunsaturated lipids of skin microsomes were susceptible to peroxidation. The chemical agents known to protect against the toxic effects of singlet oxygen and hydrogen-peroxide inhibited microsomal lipid peroxidation which suggests that these moieties may be important in mediating the reaction. Enzymic reduction of oxygen in the presence of NADPH in epidermal microsomes resulted in the formation of reactive oxygen species which initiate peroxidative deterioration of membrane lipids in epidermis. This membrane damage can be offset by a number of homeostatic cellular defense mechanisms including that of catalase and singlet oxygen quenchers. [Description provided by NIOSH]
• Subjects:
  Dermatitis Laboratories Lipids Occupational Health Safety Skin
• Keywords:
  In-vitro-studies Laboratory-animals Lipid-disorders Microsomal-enzymes NIOSH-Grant NIOSH-Publication Skin-exposure Tissue-disorders
• ISSN:
  0006-291X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  105
• Issue:
  2
• NIOSHTIC Number:
  nn:00187007
• Citation:
  Biochem Biophys Res Commun 1982 Mar; 105(2):546-552
• Contact Point Address:
  Medicine Cleveland V a Hospital 10701 East Blvd Cleveland, Ohio 44106
• Federal Fiscal Year:
  1982
• Performing Organization:
  Case Western Reserve University, Cleveland, Ohio
• Peer Reviewed:
  True
• Start Date:
  19800929
• Source Full Name:
  Biochemical and Biophysical Research Communications
• End Date:
  19830831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1c9008e3147ec9353e73a5370bad5dceb6306821c06f696133694e66c345049dd1ce04167a349f44ce39a3a1ba0a115626bca19ebcafabc6a08f32c4d08b0f69
• Download URL:
  https://stacks.cdc.gov/view/cdc/204712/cdc_204712_DS1.pdf
• File Type:
  [PDF - 404.47 KB ]