Computer-based image analysis of cartilage differentiation in embryonic limb bud micromass cultures

Details

• Personal Author:
  Garrison JC ; Peterson P ; Uyeki EM
• Description:
  An analysis was made of the effects of benzamide (55210) (BAM), sodium-butyrate (156547) and dimethyl-sulfoxide (67685) (DMSO) on chondrogenesis in embryonic chick limb bud cells in culture using a computer imaging system. A micromass culture technique was used to prepare the cultures from stage 23/24 White-Leghorn-chicken embryos. There was a direct relationship between the degree of chondrogenesis and the amount of Alcian-blue staining. Low magnification images of individual micromass colonies were converted into derived images based on their percentage transmittance relative to the background. The derived images were analyzed for relative degree of chondrogenesis using as descriptors the area and integrated optical density (IOD). IOD was the preferred descriptor due to its accuracy over a wide range of threshold values. BAM markedly enhanced chondrogenesis in culture. Sodium-butyrate markedly inhibited chondrogenesis and DMSO produced mixed results. The authors suggest that the same descriptors may be used in quantifying data from various objects which can be stained in a selected and quantitative fashion. [Description provided by NIOSH]
• Subjects:
  Amides Cells, Cultured Cytotoxins Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Author Keywords: Cartilage Cell-cultures Chick Computer Cytotoxic-effects Developmental-disorders Embryo Grants-other Image Analysis In-vitro-studies Micromass NIOSH-Grant NIOSH-Publication Skeletal-system-disorders

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• ISSN:
  0022-2720
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Kansas ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  353-361
• Volume:
  156
• NIOSHTIC Number:
  nn:00214033
• Citation:
  J Microsc 1989 Dec; 156(Pt 3):353-361
• Contact Point Address:
  Pharmacology University of Kansas 39Th St at Rainbow Blvd Kansas City, Kansas 66103
• CAS Registry Number:
  Benzamide (CAS RN 55-21-0) ; Dimethyl sulfoxide (CAS RN 67-68-5) ; Sodium butyrate (CAS RN 156-54-7)
• Federal Fiscal Year:
  1990
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other
• Performing Organization:
  University of Kansas Col Hlth Sci & Hosp, Kansas City, Kansas
• Peer Reviewed:
  True
• Part Number:
  3
• Start Date:
  19830930
• Source Full Name:
  Journal of Microscopy
• End Date:
  19940831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3a4db49de7b592e835b5862cbb7eda05102b5d2133b12995b5f5c100fdd3f9990c0dbed0f0a36b6fd5209cc0e8f1ee382ceaffb154335308ba8845e22cd25b9b
• Download URL:
  https://stacks.cdc.gov/view/cdc/204390/cdc_204390_DS1.pdf
• File Type:
  [PDF - 627.05 KB ]