Clastogenic and aneuploidogenic effects of cigarette smoke condensate, mitomycin C and vincristine sulfate

Details

• Personal Author:
  Channarayappa-JNa ; Ong T
• Description:
  Immunofluorescent staining of kinetochores (KC) in cytokinesis blocked Chinese-hamster-V79 cells fixed in-situ followed by chemical treatment was used to determine the clastogenic and aneuploidogenic effects of cigarette smoke condensate (CSC) as a test compound and mitomycin-C (50077) and vincristine-sulfate (2068782) as model compounds. The findings demonstrated that high concentrations of CSC are toxic to V79 cells. CSC was effective in the induction of micronucleus (MN) formation in this cell line. Data from immunofluorescent staining of MN indicated that CSC induces both KC+ and KC- micronucleated cells in a dose dependent fashion, suggesting that CSC contains both clastogenic and aneuploidogenic factors. The higher incidence of KC+ micronucleated cells compared to KC- micronucleated cells in the total micronucleated cells suggested a significant aneuploidy inducing effect of CSC. The authors note, however that since there are over 3,800 chemicals including at least 40 known carcinogens in tobacco smoke, it will not be easy to determine what chemical or chemical classes are causing clastogenic and aneuploidogenic activities. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Cytotoxins DNA Occupational Health Safety Smoke Tobacco Products
• Keywords:
  Cell-cultures Clastogens Combustion-products Cytotoxic-effects DNA-damage Genotoxic-effects In-vitro-study Mammalian-cells NIOSH-Author Tobacco-smoke
• ISSN:
  0267-8357
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  457-460
• Volume:
  7
• Issue:
  6
• NIOSHTIC Number:
  nn:00232400
• Citation:
  Mutagenesis 1992 Nov; 7(6):457-460
• CAS Registry Number:
  Mitomycin C (CAS RN 50-07-7) ; Vincristine sulfate (CAS RN 2068-78-2)
• Federal Fiscal Year:
  1993
• Peer Reviewed:
  True
• Source Full Name:
  Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b00d40495ca87a0a8d3d13bcd8a3a40947506cf22f6d7a0b78c0f90b0f5f6e0969406c4b40d9d2b6bcacdb26d26ebb1c970df07932259e477863b9afeb30aa62
• Download URL:
  https://stacks.cdc.gov/view/cdc/204337/cdc_204337_DS1.pdf
• File Type:
  [PDF - 1.57 MB ]