Cell-mediated immunity to soluble and particulate inhaled antigens

Details

• Personal Author:
  Burrell R ; Hill JO
• Description:
  Cell mediated immunity to soluble and particulate inhaled antigens was studied in guinea-pigs. English-White-guinea-pigs were exposed by inhalation to aerosols of soluble or particulate human-serum- albumin (9048468) (HSA) 1 hour every fourth day for up to 4 weeks. Soluble HSA was a 200 micrograms per milliliter solution of HSA in phosphate buffered saline, pH 7.2. Particulate HSA was HSA conjugated to respirable carboxylated latex beads. Controls were unexposed or animals inhaling unconjugated latex beads only. Selected animals were killed at weekly intervals and the lungs were removed and lavaged. Lavage fluid total and differential cellularity was determined. Lymphoid cells were recovered from the lavage fluid and tested for their ability to proliferate in response to phytohemagglutinin (PHA) and concanavalin-A (conA). Peripheral blood lymphocytes were collected and also tested for their ability to respond to PHA and conA. The indirect macrophage migration inhibition (MMI) and the transformation by antigen stimulation (TBAS) tests were also performed. The largest number of free lung cells was obtained from guinea-pigs exposed to particulate HSA, followed by those exposed to unconjugated latex beads, soluble HSA, and those unexposed in that order. The cells consisted primarily of macrophages and lymphocytes in each case. Only pulmonary lymphocytes from guinea-pigs exposed to soluble HSA showed a significant response to PHA or conA. Peripheral blood lymphocytes from all groups reacted to PHA and conA. No consistent differences between the groups were seen because of great inter animal variability. Only guinea-pigs exposed to particulate HSA consistently responded in the TBAS and MMI assay. Some soluble HSA exposed guinea-pigs showed a positive response in the MMI test after 2 weeks. When lavage cells with depleted macrophage populations were used, the response in the MMI test was decreased. The authors suggest that alveolar macrophages at in-vivo concentrations can modulate the reactivity of pulmonary lymphocytes to mitogens and antigens. The antigenicity of inhaled antigens may depend on the type and nature of cells responding to the stimulus. [Description provided by NIOSH]
• Subjects:
  Blood Immune System Laboratories Lung Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Blood-cells Immunology In-vitro-studies In-vivo-studies Inhalation-studies Laboratory-animals Lung-cells NIOSH-Grant NIOSH-Publication Particulates Physiological-response Pulmonary-system-disorders

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• ISSN:
  0009-9104
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  332-341
• Volume:
  38
• Issue:
  2
• NIOSHTIC Number:
  nn:00186814
• Citation:
  Clin Exp Immunol 1979 Nov; 38(2):332-341
• Contact Point Address:
  Microbiology West Virginia University Med C Department of Microbiology Morgantown, W VA 26506
• CAS Registry Number:
  Blood serum albumins (CAS RN 9048-46-8)
• Federal Fiscal Year:
  1980
• NORA Priority Area:
  Pulmonary System Disorders
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  True
• Start Date:
  19710601
• Source Full Name:
  Clinical and Experimental Immunology
• End Date:
  19830831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1a2599da9e329d66bef45efde0c3815d3c99f3282c1ec2984dac37f4b7aeec477f877fe97d349910406dba20454146c4f906933fd0acde7a0070a3c64f21d6c7
• Download URL:
  https://stacks.cdc.gov/view/cdc/204295/cdc_204295_DS1.pdf
• File Type:
  [PDF - 1.24 MB ]