Binding of halothane-free radicals to fatty acids following UV irradiation

Details

• Personal Author:
  Bösterling B ; Trevor A ; Trudell JR
• Description:
  An attempt was made to establish the existence of halothane free radicals and determine their structure by two techniques. Halothane (151677) was first irradiated with ultraviolet light in the presence of methyl-oleate. If a halothane free radical was present it would be expected to add preferentially to the double bond of methyl- oleate to produce a substituted saturated methyl stearate, for example, with the 1-chloro-2,2,2-trifluoroethyl substituent added to either the 9 or 10 position of the fatty acid chain. The authors also endeavored to trap halothane free radicals by exposing halothane vapor diluted in argon to an ultraviolet light source and then bubbling it into a solution of a free radical spin trap. This spin trap, phenyl-t-butylnitrone was able to react rapidly with free radicals and retain the unpaired electron for a time sufficient to observe a free radical spectrum with an electron paramagnetic resonance spectrometer. If the initial radical spin trap adduct were stable, hyperfine splittings in the electron paramagnetic resonance spectrum would allow interpretation of the structure of the free radical. The results indicated that the addition of a 1- chloro-2,2,2-trifluoroethyl radical to the double bond of a fatty acid chain was a reasonable hypothesis for the initiation of halothane metabolite binding to phospholipids and subsequent damage to the endoplasmic reticulum. The authors urged caution in the use of ultraviolet light or other ionizing radiation in the presence of halothane. [Description provided by NIOSH]
• Subjects:
  Anesthesia Energy Metabolism Halothane Hazardous Substances Occupational Health Radiation Respiratory Tract Diseases Safety Ultraviolet Rays
• Keywords:
  Anesthetics Author Keywords: Anesthetics Biotransformation Fluorometabolites Grants-other Halogenated-hydrocarbons Halothane Hepatic Lipid Peroxidation Liver Membrane Metabolic-study Metabolites NIOSH-Grant NIOSH-Publication Phospholipids Pulmonary-system-disorders Radiation-hazards Toxicity Ultraviolet-radiation Volatile

  More +
• ISSN:
  0003-3022
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  380-384
• Volume:
  56
• Issue:
  5
• NIOSHTIC Number:
  nn:00190466
• Citation:
  Anesthesiology 1982 May; 56(5):380-384
• Contact Point Address:
  Anesthesia Stanford University Department of Anesthesia Stanford, Calif 94305
• CAS Registry Number:
  (±)-Halothane (CAS RN 151-67-7)
• Federal Fiscal Year:
  1982
• NORA Priority Area:
  Other Occupational Concerns ; Grants Other ; Pulmonary System Disorders
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19800901
• Source Full Name:
  Anesthesiology
• End Date:
  19901130
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e1d908cd4548a0ec69158a1beb1b2a8fe01a07ed0ec1576e549e40b4730eca2222aeea05b1ba8a871b28a8dae7d86a3c03a54e5684e567912d377e81c387222f
• Download URL:
  https://stacks.cdc.gov/view/cdc/204234/cdc_204234_DS1.pdf
• File Type:
  [PDF - 3.94 MB ]