Binding characteristics of ortho-toluidine to rat hemoglobin and albumin

Details

• Personal Author:
  Booth-Jones AD ; Cheever KL ; DeBord, D. Gayle ; Swearengin TF ; Wissinger LA
• Description:
  Male Sprague-Dawley-rats were dosed with carbon-14 labeled ortho- toluidine (95534) (OT) at intraperitoneal doses of 10, 20, 40, 50, or 100mg/kg and sacrificed at 2, 4, 8, 18, 24, 48, or 72 hours or 7, 14, or 28 days following dosing. Peak binding for albumin (Alb) was recorded at 50mg/kg at 4 hours. The peak binding for hemoglobin (Hb) was recorded at 24 hours at the 100mg/kg dose level. OT-Hb binding appeared to increase linearly in a dose dependent manner, while the OT-Alb binding was not linear. OT bound to Alb or Hb demonstrated half lives of 2.6 and 12.3 days, respectively. A two fold increase in radioactivity bound to Hb was observed after intraperitoneal administration of 10mg/kg OT when compared to the binding observed following oral dosing. Pretreatment with phenobarbital caused an increase in OT-Hb binding. This was not noted following pretreatment with beta-naphthoflavone. The authors conclude that OT binds to both Alb and Hb and that a linear dose relationship exists for Hb. The biological half lives for the protein adducts were several times those reported for elimination of OT or its metabolites via the urine. This provides evidence that these proteins may be valuable biomarkers of exposure to OT in the occupational setting. [Description provided by NIOSH]
• Subjects:
  Amines Antioxidants Coloring Agents Hematologic Tests Laboratories Occupational Health Safety
• Keywords:
  Biological-monitoring Blood-analysis In-vivo-study Laboratory-animals NIOSH-Author Pigments Protein-chemistry Protein-synthesis
• ISSN:
  0340-5761
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  231-236
• Volume:
  66
• Issue:
  4
• NIOSHTIC Number:
  nn:00207189
• Citation:
  Arch Toxicol 1992 Apr; 66(4):231-236
• CAS Registry Number:
  o-Toluidine (CAS RN 95-53-4)
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  True
• Source Full Name:
  Archives of Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:90edab4f59f4499bb8caed70a92e285ce331bec91cc5fed989811d7876285ea153684a26018cbeb2897522862524880cf7a41821a2f8fe9e25cffc548586a7cc
• Download URL:
  https://stacks.cdc.gov/view/cdc/204231/cdc_204231_DS1.pdf
• File Type:
  [PDF - 491.91 KB ]