Alterations in alveolar type II cell metabolism induced by tetrandrine and other alkaloids

Details

• Personal Author:
  Bowman L ; Ma JK ; Ma JY ; Miles PR
• Description:
  Cellular and mitochondrial ATP production and oxygen consumption were examined in isolated rat alveolar type-II cells following exposure to the bisbenzylisoquinoline alkaloid tetrandrine (518343) (TT), which has been used in treating fibrotic lung diseases in China. Also examined was the question as to whether or not intracellular calcium levels may be important in the TT observed effects. Alveolar type-II cells were isolated from the lungs of male Sprague-Dawley-rats, and tests were conducted in untreated cells and in cells exposed to bisbenzylisoquinoline alkaloids, the oxidative phosphorylation uncoupler carbonyl-cyanide-m- chlorophenylhydrazone or 4-bromo-A-23187 (a calcium ionophore). Results indicated that TT and other bisbenzylisoquinoline alkaloids are able to change the metabolism of isolated alveolar type-II cells. The most significant effect appeared to be a reduced level of cellular ATP in the exposed cells. This may be caused by an alkaloid induced increase in the intracellular calcium concentration. The authors suggest that the increased internal calcium may result in a reduction in mitochondrial ATP formation with no effect on mitochondrial oxygen consumption. The overall effect is a reduced cellular ATP level and subsequent impaired ability of type-II cells to perform normal functions. [Description provided by NIOSH]
• Subjects:
  Biopharmaceutics Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Alveolar-cells In-vitro-studies Laboratory-animals NIOSH-Author Pharmaceuticals Pulmonary-system Respiratory-system-disorders Therapeutic-agents
• ISSN:
  0041-008X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  142-149
• Volume:
  119
• Issue:
  1
• NIOSHTIC Number:
  nn:00232157
• Citation:
  Toxicol Appl Pharmacol 1993 Mar; 119(1):142-149
• CAS Registry Number:
  Tetrandrine (CAS RN 518-34-3)
• Federal Fiscal Year:
  1993
• NORA Priority Area:
  Pulmonary System Disorders
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Applied Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ed31c72eaf1fd0e654cd9109ef3094a85c4a25028fb2106663e0aedb007855e126a03483f71a269f5af900375a99637cd7ad3bb17974845d97c07f42fed364fd
• Download URL:
  https://stacks.cdc.gov/view/cdc/204106/cdc_204106_DS1.pdf
• File Type:
  [PDF - 758.95 KB ]