A biological monitoring method for o-toluidine and aniline in urine using high performance liquid chromatography with electrochemical detection

Details

• Personal Author:
  Brown KK ; Simon S ; Teass AW ; Ward, Elizabeth M.
• Description:
  A method for the determination of o-toluidine (95534) and aniline (62533) in the urine of workers occupationally exposed to amines was described. Urine samples were hydrolyzed by sodium-hydroxide, extracted with butyl-chloride and hydrochloric-acid, and the concentrations of the two amines in the aqueous phases were determined by high performance liquid chromatography and colorimetric electrochemical detection. The average recoveries of o- toluidine and aniline from field samples were 91% and 100%, the precision was 13% and 16% relative standard deviation, and the limits of detection were 0.6 and 1.4 micrograms/liter, respectively. Method performance was assessed by analyzing 171 specimens acquired during the field investigation. Analysis of urine specimens from chemical factory workers by this method demonstrated median preshift values for o-toluidine for exposed workers of 11 micrograms/liter and for nonexposed workers of 0.7 micrograms/liters. The median postshift value for exposed workers was 65 micrograms/liter, while that for nonexposed workers was 2.6 micrograms/liters. The median values seen for aniline were 11 micrograms/liter for preshift exposed, 2.0 micrograms/liter for preshift nonexposed, 23 micrograms/liter for postshift exposed, and 3.2 micrograms/liter for postshift nonexposed. The ruggedness of the method was tested by variations of eight procedural steps. The authors conclude that a significant uptake and accumulation of these amines occurred during and across workshifts based on the results obtained from the method. [Description provided by NIOSH]
• Subjects:
  Chemistry Laboratories Manufacturing And Industrial Facilities Occupational Exposure Occupational Health Polycyclic Aromatic Hydrocarbons Safety Urine
• Keywords:
  Analytical-methods Aromatic-hydrocarbons Biological-monitoring Chemical-analysis Chemical-industry-workers Laboratory-techniques NIOSH-Author Occupational-exposure Urinalysis
• ISSN:
  1047-322X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences ; DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  10
• Issue:
  6
• NIOSHTIC Number:
  nn:00227370
• Citation:
  Appl Occup Environ Hyg 1995 Jun; 10(6):557-565
• CAS Registry Number:
  Aniline (CAS RN 62-53-3) ; o-Toluidine (CAS RN 95-53-4)
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  True
• Source Full Name:
  Applied Occupational and Environmental Hygiene
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:868c1f810320bfb228ec3454777a3e747ca0cd822a41ff97957c04a4ec5523fd5a04566c24ceb330a592bc4818d3718413cb8e92fdc8f6b303f1cd0f37f84e11
• Download URL:
  https://stacks.cdc.gov/view/cdc/203974/cdc_203974_DS1.pdf
• File Type:
  [PDF - 1012.03 KB ]