4,4'-Methylene-bis(2-chloroaniline)-DNA adduct analysis in human exfoliated urothelial cells by 32P-postlabeling

Details

• Personal Author:
  DeBord, D. Gayle ; Kaderlik KR ; Kadlubar FF ; Osorio, Ana Maria ; Talaska G
• Description:
  DNA adduction in urothelial cells of a person exposed to 4,4'- methylene-bis(2-chloroaniline) (101144) (MOCA) was studied. Urine samples were collected from a 30 year old male worker 4, 18, 24, 44, 49, 73, 98, 138, 145, 215, 354, 399, 407, and 408 hours after he was accidentally exposed to molten MOCA. Urothelial cells were collected by filtering the samples through 5 micrometer pore size cellulose nitrate filters. The DNA was extended and analyzed for DNA/MOCA adducts by phosphorus-32 post labeling and thin layer chromatography. A single adduct that cochromatographed with N- (deoxyadenosine-8-yl)-4-amino-3-chlorobenzyl-alcohol was detected in urine samples collected 4 to 98 hours after exposure. The maximum adduct concentration was 516 adducts/10(8) nucleotides occurred 4 hours post exposure. At the 18 hour sampling point, the adduct concentration had decreased five fold compared to the concentration at 4 hours. At 98 hours the adduct concentration was 6 adduct/10(8)nucleotide. The authors conclude that the results suggest that MOCA may be genotoxic and supports the hypothesis that MOCA is a potential bladder carcinogen. [Description provided by NIOSH]
• Subjects:
  Biochemistry Case Reports Neoplasms Occupational Exposure Occupational Health Safety Urine Urogenital Diseases Urogenital System
• Keywords:
  Biochemical-indicators Bladder-cancer Case-studies Chlorinated-anilines DNA-adducts Molecular-biology NIOSH-Author Occupational-exposure Urinalysis
• ISSN:
  1055-9965
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  63-69
• Volume:
  2
• Issue:
  1
• NIOSHTIC Number:
  nn:00217356
• Citation:
  Cancer Epidemiol Biomark Prev 1993 Jan; 2(1):63-69
• CAS Registry Number:
  3,3′-Dichloro-4,4′-diaminodiphenylmethane (CAS RN 101-14-4)
• Federal Fiscal Year:
  1993
• Peer Reviewed:
  True
• Source Full Name:
  Cancer Epidemiology, Biomarkers & Prevention
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eecddf84308a1a17cdfbfb149abe3efb13069263f660eed836c63daeb59ee98e405393ea48c0b9bbf2196b8660f50715b2d2c48e9b4c703859690e06cf50bfa1
• Download URL:
  https://stacks.cdc.gov/view/cdc/203973/cdc_203973_DS1.pdf
• File Type:
  [PDF - 1.36 MB ]